摘要
以牡丹品种赵粉为试材,采用RT-PCR和RACE方法从雄蕊中获得了一个牡丹苹果酸脱氢酶基因cDNA全长,命名为PsMDH,GenBank登录号为HQ449567。其cDNA全长1 283 bp,包含80 bp的5'非编码区、203 bp的3'非编码区和一个长度为999 bp编码332个氨基酸的开放阅读框。序列比对和系统进化分析表明,PsMDH与毛果杨的亲缘关系最近,相似性达93.37%以上。相对荧光定量PCR分析表明,PsMDH在心皮中的表达量最高,在雄蕊中表达量最低。成功构建植物表达载体,为牡丹品种改良提供基础。
In this work,a full-length cDNA sequence of MDH gene was obtained from petals of tree peony（Paeonia suffruticosa L.cv.Zhao Fen）using RT-PCR and RACE,named PsMDH（GenBank accession No.HQ449567）.The full length of PsMDH cDNA is 1 283 bp,containing a 5′-untranslated region（5′-UTR） of 80 bp,a 3′-UTR of 203 bp,and an opening reading frame（ORF） of 999 bp encoding a 332 predicted amino acids.Subsequently,sequence comparison and phylogenetic analysis revealed that PsMDH shared more than 93.37% homology with Populus trichocarpa.Relative Real-time PCR analysis indicated that PsMDH showed the highest transcript abundance in carpels and the lowest levels in stamens.Construction of plant expression vector lay a foundation for variety improvement in tree peony.
出处
《华北农学报》
CSCD
北大核心
2011年第4期32-37,共6页
Acta Agriculturae Boreali-Sinica
基金
国家“863”项目(2006AA100109)
关键词
牡丹
苹果酸脱氢酶基因克隆
表达
载体构建
Tree peony
Cloning of malate dehydrogenases
Bioinformatics analysis
Expression
Construction of vector
