摘要
基因OsBRR1是LRR-RLKs基因家族中的一员,LRR-RLKs的成员大多参与调控植物的生长发育以及防卫反应。本研究分别对OsBRR1的转基因干涉株系和超表达株系进行了稻瘟病抗病鉴定。结果显示,转基因干涉株系比转空载体和野生型对照株系对具有中等毒性的稻瘟病菌株研54-04显示更强的感病性;而超表达株系对三种不同的强致病力菌株97-27-2、99-31-1与中10-8-14具有显著的抗性。结果表明,OsBRR1的表达与水稻对稻瘟病的抗性密切相关,参与调控水稻对稻瘟病的抗性。
OsBRR1 is a novel member of leucine-rich repeat receptor-like kinases family,most of which regulate a wide variety of developmental and defense-related processes.In this study,evaluation of disease resistance was performed separately to the lines of OsBRR1-suppression plants as well as overexpressing plants.Wild-type plants showed intermediate resistance to weakly virulent isolate of Magnaporthe oryzae,Ken 54-04,while OsBRR1-suppression plants were susceptible to Ken 54-04.Furthermore,OsBRR1-overexpressing plants exhibited enhanced resistance to some virulent isolates（97-27-2,99-31-1 and Zhong 10-8-14）.These results indicate that OsBRR1 is involved in rice resistance responses to blast fungus and mediates resistance to rice blast.
出处
《华北农学报》
CSCD
北大核心
2011年第4期27-31,共5页
Acta Agriculturae Boreali-Sinica
关键词
类受体激酶
稻瘟病抗性
RNA干涉
超表达
Receptor-like kinase
Rice blast resistance
RNA interference
Overexpression
