摘要
采用酶联免疫吸附测定法(Enzyme-linked immunosorbent assay,ELISA)测定玉米粗缩病发病植株中,不同病级植株内源激素水平。结果表明,不同病级玉米植株中,赤霉素(Gibberellin GA3)、生长素(Indole-3-acetic acid,IAA)、脱落酸(Abscisic acid,ABA)和玉米素(Zeatin,ZR)的含量变化特点不同:人工接种RBSDV后,病株中GA3及ZR含量明显低于健株,且随病级增加,植株体内GA3、ZR含量逐渐降低,其含量与病情严重度呈显著正相关;抽穗孕穗期病株叶片中IAA含量低于健株,但根部IAA的含量差异不明显;人工接种RBSDV后,病株中ABA含量明显升高,且随病情严重度增加,含量逐渐升高,其含量与病情严重度呈显著负相关。
ID-ELISA for detecting the endogenous hormone contents was adopted to find out the correlation of the symptoms and the endogenous hormones.In the study,high-susceptble corn variety Zhengdan 958 was inoculated of RBSDV onto the seedlings through the virulent vectors of Laodelphax striatellus Fallen in greenhouse.The four tested endogenous hormones were gibberellin（GA3）,indole-3-acetic acid（IAA）,abscisic acid（ABA） and zeatin riboside（ZR）.The results were summaried as follows：in leaves and roots,GA3 contents in infected plants were obviously lower than those in the healthy,besides the more stunted plants were,the lower GA3 contents detected;while ABA contents kept higher in diseased than in healthy ones through the whole growth stages.Moreover,ABA contents increased more as the symptoms developed.IAA contents showed higher at the beginning and forming periods of enations（seedling to jointing stages） in the diseased than in the healthy controls.By big trumpet growth stage,it showed no obvious difference between diseased and healthy ones.There were higher ZR in levels and roots in healthy than in diseased ones.In young shoot tissue,the contents of four kinds of endogenous hormones were no significant difference in heathy and infected plants in seedling stage.It indicated that the symptoms appearance and development were strongly correlated to the endogenous hormone contents.
出处
《华北农学报》
CSCD
北大核心
2011年第6期217-220,共4页
Acta Agriculturae Boreali-Sinica
基金
河北省自然科学基金资助项目(C2009001323)
国家科技支撑计划(2006BAD02A16-3)
关键词
水稻黑条矮缩病毒
内源激素
玉米粗缩病
玉米
Rice black-streaked dwarf fijivirus（RBSDV）
Endogenous hormone
Maize rough dwarf disease
Maize
