摘要
根据牛Y染色体特异重复序列设计合成5对牛Y染色体特异引物,依据牛微卫星基因设计合成3对牛染色体内标引物。每对引物分别扩增牛基因组DNA,筛选出2对牛Y染色体特异引物和1对牛DNA特异内标引物。通过对多重PCR扩增条件进行优化组合,建立1个可用于牛早期胚胎性别鉴别的多重PCR方法及引物组合:BY1/A1。
Basing on the sequence of bovine Y-specific repeat sequence,5 pairs of bovine male-specific PCR primers were designed,meanwhile basing on the microsatellite genes,3 pairs of bovine-specific PCR primers were designed as internal control primers.Two pairs primers of Y-specific and 1 pairs of bovine-specific were selected basing on the single PCR amplification of bovine DNA,one pairs of primer combinations BY1/A1 was established for sex identification by Multi-PCR amplification of bovine genomic DNA and embryos.
出处
《华北农学报》
CSCD
北大核心
2011年第6期89-93,共5页
Acta Agriculturae Boreali-Sinica
基金
2010年省财政专项(2010055008)
河北省自然科学基金项目(C2009001308)
关键词
牛
性别鉴别
多重PCR
胚胎
Bovine
Sex identification
Multi-PCR
Embryos
