摘要
脯氨酸在提高植物抗逆性方面起着非常重要的作用。本研究以朝鲜碱茅茎叶组织提取的RNA为模板,根据已报道的P5CS基因同源序列设计引物,通过RT-PCR法扩增出1个P5CS的cDNA序列,全长2 158 bp,是一个完整开放阅读框,编码含716个氨基酸的蛋白,Genebank登陆号为:HQ637435。与其他植物P5CS基因进行同源性比对的结果显示,朝鲜碱茅P5CS基因的核苷酸和氨基酸序列与小麦的同源性最高。并对其信号肽、疏水性、跨膜结构、二级结构和主要功能域做了预测。半定量RT-PCR结果表明,PuP5CS基因在朝鲜碱茅的根部和叶片均有表达,但是根部的表达量较低,叶片中的表达量较高,在4种胁迫处理条件下,表达规律也不尽相同。
Delta 1-pyrroline-5-carboxylate synthetase（P5CS）plays a critical role in improving the stress tolerance of plants.In this study,the full length cDNA sequence of P5CS gene was cloned from Alkaligrass（Puccinellia chinampoensis）leaves using RT-PCR method,the primers were designed according to the homologous P5CS gene sequences of other plant species.Sequence analysis showed that the nucleotide sequence of this gene is 2 158 bp,containing a complete open reading frame and encoding 716 amino acids,Genebank：HQ637435.Nucleotide and amino acid sequence analysis revealed that PuP5CS shares high identity with the orthologs from Triticum aestivum.And its signal peptide,hydrophobicity/hydrophilic,trans-membrane domain,secondary structure and main functional domains were predicted.Semi-quantitative RT-PCR analysis showed that PuP5CS expressed in different tissues,but the expression in root was lower,and in leaf much higher.Various elevated levels of PuP5CS expression have been detected when exposed to 4 different stress experimental treatment,and the results was not the same.
出处
《华北农学报》
CSCD
北大核心
2011年第6期20-26,共7页
Acta Agriculturae Boreali-Sinica
基金
国家牧草产业技术体系
