摘要
目的探讨肝动脉化疗栓塞联合重组人血管内皮抑制索对肝癌生长和转移的抑制。方法经皮穿刺瘤块推送法建立VX2肝癌模型。将建模成功的60只荷瘤兔随机分为3组：G1组接种后第2l天行TACE，术后每天静脉推注生理盐水，连续7d；（32组接种后第2l天行TACE，术后每天静脉推注重组人血管内皮抑制素．连续7d。G3组接种后第21天行肝动脉造影，术后每天静脉推注生理盐水，连续7d。比较各组干预后体积与干预前体积之比（V2／V1）、肿瘤转移率、微血管密度（MVD）的差异。结果13只瘤兔因干预后7d之内死亡而被剔除，各组完成所有指标观察的瘤兔只数：14，15，18。各组平均V2／V1：1．216±0．304，1．211±O．314，4．723±1．562，单因素方差分析示差异有显著性（P=0．000），LSD法两两比较，G3组与Gl组、G3组与G2组差异有显著性，而G2组与GI组差异无显著性。各组转移率分别为：78．57％，33．33％，77．78％，卡方检验示差异有显著性（P=0．012），卡方分割示G2组与Gl组、G2组与G3组差异有显著性，而Gl组与G3组差异无显著性。各组平均MVD：75．2．4-11．0，21．7±10．1．61．34-12-3，单因素方差分析差异有显著性（P=0．000），LSD法两两比较各组差异皆有显著性（P〈0．01）。结论TACE联合重组人血管内皮抑制素治疗肝癌对肿瘤生长的抑制并不优于单纯TACE，但可抑制血管新生，抑制肿瘤转移。
Objective To investigate the inhibitory effects of transcatheter arterial chemoembolization combined with recombinant human endostatin on the growth and metastases of rabbit VX2 hepatic carcinoma. Methods Sixty New Zealand rabbits used as implanted VX2 hepatocarcinoma model were randomly divided into 3 groups.G1 ：TACE was performed on the 21^st day after inoculation,saline via intravenous injection was followed for 7 days.G2：TACE was performed on the 21^st day after inoculation,recombinant human endostatin via intravenous injection was followed for 7 days.G3：Hepatic angiography was performed on the 21^st day after inoculation,saline via intravenous injection was followed for 7 days.The differences of tumor growth（V2/ V1）,tumor metastasis rate,tumor microvessel density in each group were compared.Results Forty seven animals survived to the 28^th day after inoculation：Gl=14,G2=15,G3=18.Average V2/VI in each group were：Gl=l.216 0.304,G2=1.211= 0.314,G3=4.723 ＋ 1.562.The difference of average V2/V1 in each group was statistically significant（P=-0.000）,among which average V2/V 1 of G 1 and G2 was lower than that of G3,however,the difference between G1 and G2 was not statistically significant.The metastasis rates in each group were：Gl=78.57%（ll/14）, G2=33.33%（5/15）,G3=77.78%（14/18）.The difference in each group was statistically significant（P=0.012）,among which the metastasis rate of G2 was lower than that of G1 and G3,however the difference between G1 and G3 was not statistically significant.The MVD in each group were：G 1 =75.2 ＋ 11.0,G2=21.7 ± 10.1 ,G3=61.3 ± 12.3. The differences between each group was statistically significant（P=0.000）.Conelusion The combination of TACE and recombinant human endostatin via intravenous injection for the treatment of hepatic carcinoma didn＇t induce superior suppression on tumor growth than TACE alone,however, it could suppress angiogenesis,and cause superior suppression on tumor metastasis than TACE alone.
出处
《中国血液流变学杂志》
CAS
2011年第4期589-592,共4页
Chinese Journal of Hemorheology
