摘要
作首用白细胞抗体,观察了酵母多糖活化的血浆(ZAP)分别对白细胞数正常(n=7)和减少(n=7)的山羊肺淋巴中脂质过氧化物(LPO)及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-px)的影响,及其与肺损伤的关系。ZAP导致肺淋巴LPO含量由2.26±0.03nmol/ml增至3.26±0.09nmol/ml,SOD活性由258.10±4.53u/ml升至354.07±13.27u/ml,同时引起通透性肺损伤。而GSH-px活性则由189.34±4.16u/ml降为184.59±5.54u/ml。白细胞减少组山羊肺损伤程度较轻,LPO量增加不明显。实验结果提示:白细胞及其产生的自由基参与ZAP肺损伤过程。
The effects of zymosan-activated plasma(ZAP) on the lipid peroxides(LPO) content, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in lung lymph and their relationship to lung injury were studied in control (with normal leukocyte count) and leukopenic goats. In the control goats, after ZAP infusion, LPO production elevated, that was coincided with the increase of lung lymph flow (QL), and the clearence rate of lung lymph protein (LLPC). SOD activity was also elevated, but GSHpx activity was inhibited. In leukopenic goats, ZAP challenge caused an increase in LPO production, but its content was lower than that in control goats. No marked change was observed in QL and LLPC. The results suggest that: (1) Leukocytes and oxygen free radicals are involved in the pathogenesis of lung injury due to ZAP infusion; (2) Leukocytes play an important role in producing oxygen free radicals.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1990年第2期95-99,共5页
Chinese Journal of Pathophysiology
关键词
肺损伤
白细胞
氧自由基
ZAP
Leukopenia
Lipid peroxides
Superoxide dismutase
Glutathionc peroxidase
