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大蒜辣素对多药耐药HepG2细胞中MDR1基因表达影响 被引量：4

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摘要 目的多药耐药(MDR)是肿瘤化疗的主要障碍,该研究旨在体外建立MDR人肝癌HepG2细胞株(HepG2/mdr),并探讨大蒜辣素(Allicin)对MDR1表达的影响。方法采用高效液相色谱法(HPLC)提纯Allicin,采用间歇逐步增加阿霉素(adramycin,ADM)剂量法建立人肝癌MDR细胞株HepG2/mdr,运用细胞计数试剂盒(Cell counting kit-8,CCK-8)检测耐药细胞株耐药性,运用倒置显微镜观察不同浓度Allicin孵育下HepG2/mdr细胞形态学变化,运用逆转录聚合酶链反应(RT-PCR)及免疫印迹(Western blot)测定不同浓度Allicin作用下,HepG2/mdr细胞MDR1mRNA与蛋白表达情况。结果 Allicin经HPLC法提纯后其纯度达到99%。在2000nmol/L ADM作用下,HepG2/mdr细胞生长良好,而HepG2细胞生长停滞与死亡,且Allicin能阻止HepG2/mdr细胞生长,促进其死亡,随着Allicin浓度增加越加明显。Allicin能降低MDR1 mRNA和蛋白表达,呈现剂量依赖性。结论成功建立人肝癌MDR细胞株HepG2/mdr,Allicin能通过抑制MRD1基因的表达,恢复肿瘤细胞对化疗药物的敏感性。
作者 燕丹 王坚 袁耀佐 王钿钿
机构地区 东南大学附属第二医院 中国药科大学药学院 江苏省药品检验所 东南大学
出处 《时珍国医国药》 CAS CSCD 北大核心 2012年第1期233-235,共3页 Lishizhen Medicine and Materia Medica Research
关键词 大蒜辣素 人肝癌HEPG2细胞 多药耐药基因
分类号 R962 [医药卫生—药理学]
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参考文献17

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二级参考文献15

  • 1高世勇,王秋娟,季宇彬.龙葵碱对HepG2细胞内caspase-3及Bcl-2蛋白含量的影响[J].中国天然药物,2006,4(3):224-229. 被引量：8
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时珍国医国药
2012年 第1期

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