摘要
目的研究多奈哌齐单次给药对乙酰胆碱酯酶(AChE)活力的影响以及其与脑组织AChE活力变化的相关性。方法受试小鼠随机分为对照组和多奈哌齐组。对照组(n=8)生理盐水0.2 mL/10 g灌胃,于给药后于2 h取血、取脑;多奈哌齐组(7亚组,每组8只)于多奈哌齐灌胃后于0.5、1、2、4、8、12和24 h各时间点取血、取脑。采用AChE测定试剂盒测定全血、血浆及脑组织AChE活性,考马斯亮蓝试剂盒测定脑组织匀浆蛋白含量。结果与对照组比较,多奈哌齐各亚组全血、血浆中AChE的活力改变无统计学意义(P>0.05);各亚组脑匀浆AChE活力改变无统计学意义(P>0.05),但2 h亚组脑匀浆中AChE活力改变显著(P<0.05)。提示全血和血浆AChE活力改变与脑组织AChE活力变化并无显著关联。结论多奈哌齐0.625 mg/kg单次给药后2 h脑组织AChE活力显著降低,但对血液AChE活性无显著影响,且血液中酶活力变化与脑组织酶活力变化无显著关联。
Objective To investigate the changes of acetylcholinesterase（AChE） activity in brain, blood and plasma after single dose administration of donepezil. Methods Sixty-four mice were divided into eight groups： normal control （0.9 % NaCl, 0.2 mL/10 g, test at 2 h） and donepezil （0.625 mg/kg; test at 0.5, 1, 2, 4, 8, 12 and 24 h, respectively） groups. Blood and brain assays were collected at the fixed-point of time after oral administration. The AChE activity and the content of protein were measured by AChE test kits and protein test kits respectively. Results The AChE activity in brain of 2 h group showed significant decreased （P〈0.05）. Others showed no difference （P〉0.05）. Conclusion Single dose （0.625 mg/kg） of donepezil can reduce AChE activity in brain after oral administration 2 h, but not in blood and plasma.
出处
《世界临床药物》
CAS
2012年第2期92-95,共4页
World Clinical Drug
