摘要
目的采用自体骨髓单个核细胞植于前交叉韧带重建术中的腱-骨界面,观察其增强腱-骨愈合强度的能力。方法 24只新西兰大白兔行双侧膝关节半腱肌肌腱前交叉韧带重建术,随机选取一侧膝关节作为实验侧,胫骨侧腱-骨界面植入自体骨髓单个核细胞,对侧不植入骨髓单个核细胞作为对照侧。于术后2、4、8、12周取材,测试移植肌腱的腱-骨界面抗拉伸强度,记录拉脱或断裂负荷及重建韧带的断裂部位。结果在术后2、4、8周,重建韧带断裂部位均发生在胫骨骨道内。术后12周,对照侧有1例自肌腱部分断裂,5例从胫骨骨道拔出;实验侧有3例自肌腱部分断裂,3例从胫骨骨道拔出。术后2周,实验侧与对照侧相比,两侧断裂负荷数值比较差异无统计学意义(P>0.05);在术后4、8、12周,两侧断裂负荷数值比较差异均有统计学意义(P<0.05)。结论自体骨髓单个核细胞可以增强腱骨界面的抗拉伸强度,有利于腱-骨界的组织愈合。
Objective To explore the effects of autologous bone marrow mononuclearcells,which were transplanted in the tendon bone interface in reconstruction of anterior cruciate ligament,on enhancing the healing potential.Methods Bilateral anterior cruciate ligaments of 24 New Zealand rabbits were removed and reconstructed with autogeneic tendons of semitendinosus muscles.The autologous bone marrow mononuclearcells were injected into the bone tunnel of one side of each animal randomly as the experimental group.The other side was not injected as control.Specimens were collected at 2,4,8,12 weeks after surgery respectively for biomechanical test.The maximal pull-out loads and sites of break were recorded.Results The sites of break were all in the bone tunnels of bilateral tibia in postoperative 2,4,8 weeks.There were 3 cases of break in tendons and 3 cases of break in bone tunnels in the experimental group,and there were 1 case of break in tendon and 5 cases of break in bone tunnels in the control group.The difference of the pull-out loads between the two groups had statistical significance（P0.05） in postoperative 4,8,12 weeks,while there was no statistical significance of pull-out loads between the two groups in postoperative 2 weeks.Conclusion Autologous bone marrow mononuclearcells can enhance the tensile strength of interface of tendon and bone and benefit tendon-bone healing.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第5期463-465,共3页
Chongqing medicine
关键词
骨髓移植
交叉韧带
膝关节
腱骨愈合
bone marrow transplantation
cruciate ligament
knee joint
tendon-bone healing
