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银屑病皮损免疫病理表型特征及其体外维持 被引量：4

Immunopathological Phenotype of Psoriatic Lesional Skin and Its Maintenance in the Transwell Skin Organ Culture Model.
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摘要 目的　研究银屑病皮损免疫表型及其体外维持。方法　检测代表银屑病病理特征的蛋白分子 ,并观察培养后这些分子的变化。结果　银屑病皮损中角蛋白 16、17、转谷酰酶I、ICAM 1、HLA DR、β1整合素及OKM 5增强表达 ,培养48h后和培养前基本相同。结论　上述蛋白分子的共同表达构成了银屑病皮损的免疫表型 ,这种表型在跨膜皮肤器官模型中 48h培养可得到良好的维持。 Objective To study the immunopathological phenotype of psoriatic lesional skin and its maintenance ex vivo using tissue culture techniques.Methods Psoriasis related markers representing psoriatic pathological features were monitored immunohistochemically before and after the culturing of the skin biopsies.Results The expression of keratin 16,keratin 17,type l transglutaminase ICAM 1,HLA DR,OKM 5 and integrinβ1 was upregulated in psoriatic lesional skin,it was almost the same after culturing for 48 hours in the transwell skin organ culture model.Conclusion The expression of above proteins gives the immunopathological phenotype of lesional psoriatic skin,it can be well preserved after 48 h culturing in transwell skin organ culture model.
作者 刘玮 赵庆利 张新华 Reno Debets Errol P Prens
机构地区 解放军空军总医院皮肤科 荷兰ERASMUS大学免疫系免疫皮肤病研究室
出处 《中国皮肤性病学杂志》 CAS 北大核心 2000年第2期79-81,共3页 The Chinese Journal of Dermatovenereology
关键词 银屑病 免疫病理表型 器官培养 Psoriasis Immunopathological phenotype Organ culture
分类号 R758.630.2 [医药卫生—皮肤病学与性病学]
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参考文献12

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同被引文献12

  • 1赵庆利,刘玮,关鹏举,王伯云,肖曼西.银屑病皮损中γ干扰素的免疫组化分析[J].中华皮肤科杂志,1994,27(5):284-285. 被引量：2
  • 2Wei L, Debets R, Hegmans JJ, et al. IL-1 beta and IFN-gamma induce the regenerative epidermal phenotype of psoriasis in the transwell skin organ culture system. IFNgamma up-regulates the expression of keratin 17 and keratinocyte transglutaminase via endogenous IL-1 production. J Pathol,1999, 187: 358-364.
  • 3Leigh IM, Navsaria H, Purkis PE, et al. Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro. Br J Dermatol, 1995, 133:501-511.
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  • 6Tanaka N,Fujioka A,Tajima S,et al.Elafin is induced in epidermis in skin disorders with dermal neutrophilie infiltration:interleukin-1 beta and turnout necrosis factor-alpha stimulate its secretion in vitro[J].Br J Dermatol,2000,143(4):728 -732.
  • 7Liu H J,Moroi S,Yasumoto S,et al.Expression of elafin in extramammary Paget's discase[J].Br J Dermatol,2005,152(3):578-579.
  • 8Henriksen P A,Hitt M,Xing Z,et al.Adenoviral gene delivery of elafin and secretory leukocyte protease inhibitor attenuates NF-kappa Bdependent inflammatory responses of human endothelial cells and macrophages to atherogenie stimuli[J].J Immunol,2004,172(7):4535-4544.
  • 9Zaidi S H,You X M,Ciura S,et al.Suppressed smooth muscle proliferation and inflammatory cell invasion after arterial injury in elafin-over-expressing mlce[J].J Clin Invest,2000,105(12):1687 -1695.
  • 10Yokota T,Hunt K K,Keyomarsi K.Downregulation of elafin gene in breast cancer:A novel target[C/OL]// AACR Annual Meeting,Washington Convention Center.Washington,D.C.,April 1-5,2006 ; impact on cellular and molecular biology[2007-05-22].http://www,aacrmeetingahstracts,org/cgi/content/abstract/2006/1/1154.html.

引证文献4

二级引证文献11

中国皮肤性病学杂志
2000年 第2期

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