摘要
为探索重组绿色木霉源双功能壳聚糖酶(rCCBE)在毕赤酵母中表达的最佳诱导条件,本研究在对甲醇浓度、诱导pH、诱导温度和诱导时间这4个因素进行单因素试验基础上,运用Box-Behnken设计的响应面试验对诱导条件进行了优化。根据回归分析确定了影响rCCBE表达的因子,求得最佳诱导条件为:诱导甲醇浓度为0.92%、pH为6.26,诱导温度为28.42℃、诱导时间80.85 h,此工艺条件下双功能酶的实测壳聚糖和CMC降解活力分别为7.32 kat/mL和7.15 kat/mL,比优化前提高了76.4%。回归模型的预测值与实测值的相对误差<1%,说明该回归方程与实际情况拟合很好。
In order to optimize the conditions of expressing recombinant Trichoderma viride bifunctional chitosanase-cellulase（rCCBE） induced by methanol,the response surface methodology designed by Box-Behnken was applied based on single factor tests.The factors relating to the expression of rCCBE was obtained with method of regression analysis.The optimum inducing conditions were as follows： methanol concentration of 0.92 %,temperature of 28.42 ℃ for 80.85 h,pH 6.26.Under the above conditions,the actual chitosanase and CMCase activities of rCCBE were 7.32 kat/mL and 7.15 kat/mL,respectively.The optimal activities ware 76.4 % higher than before.The relative error was1 % between actual chitosanase activity and the activity calculated according to the regression model,which suggested that regression equation fitted well with actual condition.
出处
《食品研究与开发》
CAS
北大核心
2012年第2期177-181,共5页
Food Research and Development
基金
国家自然科学基金(No.20876068)
江苏省自然科学基金项目(BK2009734)
关键词
响应面
双功能壳聚糖酶-纤维素酶
毕赤酵母
重组表达
response surface methodology
bifunctional chitosanase-cellulase
pichia patoris
recombinant expression
