摘要
目的探讨庚型肝炎病毒合并其他肝炎病毒在血液透析患者中的感染状况。方法逆转录－套式聚合酶链反应（ＲＴ－ｎＰＣ Ｒ）检测血液透析患者血清ＨＧＶ ＲＮＡ，ＥＬＩＳＡ法检测乙肝两对半及ＨＣＶ，ＨＤＶ抗体。结果 ８０例血透患者中，ＨＧＶ阳性１５例（１８．７％），ＨＣＶ阳性１８例（２２．５％），ＨＢｓＡｇ阳性２１例（２６，２％），未检出ＨＤＶ阳性。在１５例感染ＨＧＶ的患者中，７例（４６．７％）同时感染其他肝炎病毒，其中与ＨＣＶ，ＨＢＶ二重感染者分别为３例和 ５例，与 ＨＣＶ及 ＨＢＶ三重感染者 ３例。 １２例透析工作人员中未检出上述病毒感染。有输血史的 ３３例患者中，ＨＧＶ，ＨＢＶ及 ＨＣＶ阳性百分率均显著高于无输血史患者。输血组 ＨＧＶ与 ＨＣＶ的重叠感染率，ＨＧＶ与 ＨＢＶ，ＨＣＶ的三重感染率均显著高于非输血组（Ｐ＜０．０５）。各型肝炎病毒的感染率随着透析时间增加均升高，其中ＨＢＶ各组间比较差异均有显著性（Ｐ＜０．０５）。ＨＣＶ透析１～３ ａ与透析超过５ ａ相比，差异有显著性（Ｐ＜０．０５）。结论维持性血液透析患者为ＨＧＶ高感染人群，ＨＧＶ及其与ＨＢＶ和（或）ＨＣＶ重叠感染的高发生率与输血有关。
Objective To investigate the infection and co - infection of HGV with other hepatitis viruses and the influence of transfusion and hemodialysis the on viral infection in maintenance hemodialysis patients. Methods Genome RNA of hepatitis G virus (HGV) was amplified in sera from 80 hemodialysis patients and 12 nurses of dialysis unit using a nested poly- chain reaction (PCR) assay with specific primers. HBV, HCV and HDV were detected by ELISA method.Results The number of patients with HGV, HBV, HCV and HDV were 15(18.7%),21(26.2%), 18(22.5% ) and 0, respectively. of the 15 HGV pesitive patients, 7(46.7%) were also infected with other hepatitis viruses, 3 with HCV, 5 with HBV, 3 with HBV and HCV. Hepatitia viruses were not detected in dialysis operators. The prevalence of infection and co - infection of HGV with HBV or HCV increased significantly in the transfusions group. Conclusion HGV infection is serious in hemodialysis patients. The HGV infection and co - infection with HBV or HCV in hemodialysis patients may be related to transfusion.
出处
《广东医学》
CAS
CSCD
2000年第2期123-124,共2页
Guangdong Medical Journal
