摘要
为了解肝素对脂多糖 (L PS)诱导人肾小球系膜细胞 (MC)增殖和细胞因子分泌的影响 ,作者采用四甲基偶氮唑蓝比色分析法及生物活性法 ,分别测定了人肾小球 MC增殖和细胞因子的分泌情况。结果显示 :在正常培养条件下 ,人肾小球 MC分泌一定量的白细胞介素 6 (IL- 6 )和肿瘤坏死因子 α(TNF- α)。脂多糖诱导 MC增殖及分泌 IL- 6、TNF- α。高浓度的肝素 (5 0 0 U/ml)促进 MC增殖及分泌细胞因子 ,低浓度的肝素 (5 U/ml)抑制 MC增殖及分泌细胞因子。据此 ,作者认为肝素仅在低浓度抑制 MC增殖及细胞因子分泌 。
The human glomerular mesangial cells(MC) proliferation and cytokines secretion were detected by using MTT method and biological active method so as to assess the effects of heparin on the cultured human glomerular MC induced by lipopolysaccharides (LPS). The results showed that there were basic production of interleukin 6(IL 6) and tumor necrosis factor α (TNF α)on MC in the control group without LPS, that high concentration heparin(500U/ml) increased the effects of LPS on MC, and that low concentration heparin (5U/ml) conversely inhibited the effects of LPS on MC. These data indicate that the low concentration heparin could inhibit the proliferation of MC and the secrection of IL 6 and TNF α induced by LPS, and hence provide an experimental evidence for administration of heparin in the treatment glomerular disease.
出处
《华西医科大学学报》
CSCD
2000年第1期77-79,共3页
Journal of West China University of Medical Sciences
关键词
肝素
肾小球系膜细胞
脂多糖
细胞分泌
Heparin Mesangial cell Interleukin-6 Tumor necrosis factor-α
