摘要
目的:探讨四妙勇安汤对动脉粥样硬化(AS)斑块内血管新生的影响及作用机理。方法:随机将40只日本大耳白兔分为对照组10只,实验组30只。对照组给予普通饲料,实验组利用复合因素制备AS易损斑块模型,8周时实验组随机分为模型组、辛伐他汀组、四妙勇安汤组,第24周取材。主动脉切片进行病理形态学观察,测量内膜厚度(IT)、纤维帽厚度(FCT)、纤维帽厚度与内中膜厚度比(FCT/IMT)、脂核与斑块面积比(LCA/PA);免疫组化方法检测CD31、血管内皮生长因子(VEGF)、P38 MAPK水平;RT-PCR方法检测VEGF mRNA表达。结果:与模型组比较,四妙勇安组IT降低,FCT、FCT/IMT增加;CD31、VEGF、P38MAPK水平及VEGF mRNA表达显著下降。结论:四妙勇安汤可稳定AS斑块,抑制斑块内血管新生,其机制可能与抑制斑块内VEGF和P38MAPK表达有关。
Objective： To observe the effect of Simiaoyongan Decoction（SYD） on angiogenesis in rabbit＇s Atherosclerosis（AS） plaque.Methods： 40 Japanese white rabbits were randomly divided in two groups,that is,the control group（n=10,normal diet） and the experiment group（n=30）,in which AS vulnerable plaque model was established by feeding high-fat diet,injecting Bovine Serum Albumin（BSA） and balloon catheter overstretching on abdominal aorta artery.At the 8th week,30 animals in experiment group were randomly divided in three groups,that is,model group（n=10）,SYD group（n=10）,simvastatin group（n=10）.The rabbits were sacrificed at the 24th week.Pathological changes were observed by HE,oil red O and Masson staining,and then measured intimae thickness（IT）,fiber cat thickness（FCT）,FCT/IMT,lipid core area to plaque area ratio（LCA/PA）.CD31,VEGF,P38 MAPK protein were detected with immunohistochemistry（IHC）,and VEGF mRNA signal was assessed by polymerase chain reaction（PCR）.Results： IT in SYD group was lower and FCT,FCT/IMT levels were higher than that of the model group.Compared with the model group,the levels of CD31,VEGF,P38MAPK in the SYD group decreased,and VEGF mRNA expression was inhibited by SYD（P0.01or P0.05）.Conclusion： SYD could suppress angiogenesis in AS plaque to stabilize AS vulnerable plaque.It may act by decreasing P38 MAPK,VEGF protein and VEGF mRNA expressions.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2012年第2期161-163,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
教育部科学技术研究重点项目资助(206009)
国家自然科学基金项目(30901890)
天津市自然科学基金重点资助项目(09JCZDJC20700)
关键词
四妙勇安
AS易损斑块
血管新生
Si miao yong＇an Decoction
angiogenesis
atherosclerosis plaque
