摘要
目的探讨酪氨酸激酶受体B（TrkB）、三结构域蛋白29（TRIM29）在胃癌患者术前胃镜下胃黏膜活检组织中的表达对胃癌淋巴结转移的预测价值。方法选取2005至2009年于北京大学第三医院普外科行胃癌根治术，术前曾于消化科行胃镜活检的病例，共67例，其中男39例，女28例，应用免疫组织化学染色的方法检测TrkB、TRIM29在术前胃镜下活检组织中的表达，探讨其与胃癌淋巴结转移及其他临床病理变量的关系。应用ImageproPlus6．0软件分析染色图片的吸光度（A）值，数据经对数化处理，应用SPSS17．0软件进行统计学分析。结果TrkB在胃癌有淋巴结转移42例中的表达高于无淋巴结转移25例（1gA值：4．794-0．42比3．984-0．71，t=一5．873，P：0．003）；TRIM29在胃癌有淋巴结转移36例中的表达也高于无淋巴结转移24例（5．214-0．27比5．024-0．37，t=一2．257，P=0．040）。多因素回归分析显示TrkB是胃癌淋巴结转移的独立影响因素。根据实验结果建立判别式[判别值=1．705×TrkBlgA值＋1．803×TRIM29]gA值-16．880，截断值=01，根据判别式预测淋巴结转移的准确度达80．O％（48／60）。阳性似然比3．332，阴性似然比0．223。TrkB高表达组总体生存率低于低表达组[36．0％（9／25）比66．7％（16／24），P=0．047]；TRIM29高表达组总体生存率低于低表达组[38．1％（8／21）比66．7％（14／21），P=0．044]。结论术前胃镜下活检胃癌组织TrkB、TRIM29免疫组织化学表达与淋巴结转移相关，联合TrkB、TRIM29表达建立的判别式对胃癌淋巴结转移治疗前预测有重要价值。
Objective To analyze the expressions of TrkB and TRIM29 in biopsy tissues from preoperative gastroscopy in patients with gastric cancer and investigate the relationship between their expression and rate of lymph node metastasis. Methods Through immunohistochemistry, the authors analyzed the expression status of TRIM29 and TrkB in biopsy tissues from preoperative gastroscopy in 67 patients with a diagnosis of gastric cancer undergoing radical operations during 2005--2009 at Peking University Third Hospital. There were 39 males and 28 females. Then the relationship was explored between the expression of both proteins and lymph node metastasis and other clinical pathological factors. Image pro Plus 6. 0 software was used for image analysis. The data underwent a logarithmic process and were analyzed through SPSS 17.0 statistical software. Results The expression of TrkB in lymph node metastasis （ n = 42） was higher than that in without lymph node metastasis （ n = 25 ） （ lgA 4.79 ＋ 0.42 vs 3.98 ＋ 0.71, t = -5. 873, P = 0.003）, while the expression of TRIM29 with lymph node metastasis （n = 36）was also higer than that in without lymph node metastasis （ n = 24） （5.21 ± 0.27 vs 5.02 ± 0.37, t = - 2. 257, P = 0. 040）. Logistic regression analysis showed that the TrkB expression was an independent predictor for lymph node metastasis. A discriminant was set based on the result （ discriminant value = 1. 705 TrkB lgA ＋ 1. 803 TRIM29 lgA -16. 880, cutoff value = 0）. This discriminant achieved a prediction accuracy of 80.0% （48/60）, a positive likelihood ratio 3. 332 and a negative likelihood ratio 0. 223. Patients with a high expression of TrkB showed a worse survival rate than those with a low TrkB expression [ 36.0% （9/25） vs 66. 7% （ 16/24）, P = 0. 047）. Patients with a high TRIM 29 expression showed a worse survival rate than those with a low TRIM29 expression [ 38. 1% （8/21）vs 66. 7% （14/21）, P = 0. 044 ]. ConclusionThe expressions of TrkB and TRIM29 are correlated with lymph node metastasis in gastric cancer, and discriminant based on the expressions of TrkB and TRIM29 had important values for preoperative prediction of lymph node metastasis in gastric cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2012年第6期376-379,共4页
National Medical Journal of China
