摘要
[目的]建立石墨炉原子吸收光谱法测定全血中铍（Be）的方法。[方法]全血样用5%（v/v）硝酸脱去蛋白,经离心后取上清液进样,以0.5%（m/v）硝酸镁Mg（NO3）2作为基体改进剂,采用标准工作曲线法测定。[结果]测定方法的线性范围为0.18~5.00μg/L,相关系数（r）为0.9996,相对标准偏差（relative standard deviation,RSD）为2.7%~4.2%,检出限为0.27μg/L,加标回收率为90%~100%。[结论]该方法取样量少,消解处理简单,且快速、准确,适用于全血中铍的测定。
[Objective] To establish the method for determination of beryllium in whole blood with graphite furnace atomic absorbance spectrometry.[Methods] After the protein in whole blood was removed by nitric acid 5%（v/v） and centrifugal separation,it was determined by standard working curve using Mg（NO3）2 0.5%（m/v） as matrix modifier.[Results] The linearity range of the method was 0.18-5.00 μg/L,the correlation coefficient（r） was 0.999 6,the relative standard deviation（RSD） was 2.7%-4.2%,the detection limit was 0.27 μg/L,and the recovery was 90%-100%.[Conclusion] This method is characterized by less amount of samples,simple digestion,better degree of precision and accuracy.It is fit to determine beryllium in whole blood.
出处
《环境与职业医学》
CAS
北大核心
2012年第2期97-99,共3页
Journal of Environmental and Occupational Medicine
关键词
石墨炉原子吸收光谱法
全血
铍
graphite furnace atomic absorbance spectrometry
whole blood
beryllium
