丹参酮ⅡA磺酸钠对Ang Ⅱ诱导的心肌细胞氧化应激的影响被引量：12

Effects of Sodium Tanshinone ⅡA Sulfonate on Ang Ⅱ-Induced Cardiomyocyte Oxidative Stress

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摘要目的观察丹参酮ⅡA磺酸钠(sodium tanshinoneⅡA sulfonate,STS)对血管紧张素Ⅱ(angiotensin Ⅱ,Ang Ⅱ)诱导的心肌细胞氧化应激反应的影响及其机制。方法培养新生大鼠心肌细胞,建立AngⅡ诱导的心肌细胞氧化应激反应模型。用活性氧(reactive oxygen species,ROS)敏感的二氮荧光黄双乙酸盐(DCFH-DA)探针测定胞内ROS水平。酶联免疫分析法测定细胞培养物上清中8-羟脱氧鸟苷(8-OHdG)含量。以细胞存活率、细胞内超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、培养上清液乳酸脱氢酶(LDH)活性作为反应心肌细胞损伤的指标。比色法测定还原型辅酶Ⅱ(NADPH)氧化酶(NADPH oxi-dase,NOX)活性。蛋白质印迹法(Western-blot)测定NOX亚单位p47 phox表达。结果 Ang Ⅱ能显著提高心肌细胞内DCF荧光信号强度,降低细胞存活率及SOD活性,同时上调培养上清液LDH活性及MDA含量。以上氧化应激相关指标能被各浓度STS抑制。进一步研究发现,STS能抑制Ang Ⅱ上调的NADPH氧化酶活性及亚单位p47 phox表达。结论 STS可以抑制Ang Ⅱ诱导的心肌细胞氧化应激损伤反应,机制可能与下调NADPH氧化酶活性及亚单位p47 phox表达有关。 OBJECTIVE To observe the effects and mechanisms of sodium tanshinone ⅡA sulfonate（STS） on angiotensin Ⅱ（Ang Ⅱ）-induced cardiomyocyte oxidative stress.METHODS In the primary culture of neonatal rat cardiomyocytes,the content of reactive oxygen species（ROS） was measured by 2,7-dichlorofluorescein diacetate（DCFH-DA）.8-hydroxydeoxyguanosine level in the supernatant was measured by ELISA.As indexes of cardiomyocyte oxidative stress,the cellular contents of MDA and SOD,cell vialibity and LDH release were measured.NADPH oxidase（NOX） activity was measured by chromatometry.The expressions of p47 phox was assessed using Western blot.RESULTS STS can decrease Ang Ⅱ-induced elevations of ROS level and oxidative stress,and inhibit the expression of p47 phox and NOX activity.CONCLUSION The inhibitory effects of STS on Ang Ⅱ-induced cardiomyocyte oxidative stress may be associated with depressing NOX signaling pathway via down regulation of p47 phox expression.

作者杨乐邹晓静高翔李树生梁黔生杨光田

机构地区华中科技大学附属同济医院急诊内科华中科技大学附属协和医院麻醉科华中科技大学附属同济医院综合医疗科

出处《中国药学杂志》 CAS CSCD 北大核心 2012年第4期270-274,共5页 Chinese Pharmaceutical Journal

基金国家自然科学基金资助项目(8110269130901405) 湖北省自然科学基金(2011CDB538)

关键词丹参酮ⅡA磺酸钠心肌细胞肥大活性氧 NADPH氧化酶 sodium tanshinone Ⅱ A sulfonate cardiomyocyte hypertrophy reactive oxygen species NADPH oxidase

分类号 R965 [医药卫生—药理学]

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参考文献1

1杨乐,邹晓静,梁黔生,郑智.丹参酮ⅡA磺酸钠影响AngⅡ诱导心肌细胞肥大反应及p-p38,MKP-1表达[J].中国药学杂志,2007,42(20):1543-1546. 被引量：3

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