摘要
目的：研究新型重组人肿瘤坏死因子（ｒｈＴＮＦ－ＮＣ）对培养大鼠肝细胞内游离Ｃａ２＋ 浓度（［Ｃａ２＋ ］ｉ）及小鼠肝组织中Ｃａ２＋ 含量的影响。方法：以Ｆｕｒａ－２／ＡＭ 为Ｃａ２＋ 荧光检测，荧光分光光度法测定培养大鼠肝细胞内［Ｃａ２＋ ］ｉ；原子吸收分光光度法测定小鼠肝组织中Ｃａ２＋ 含量。结果：在加入ｒｈＴＮＦ－ＮＣ３ ｈ 后，可剂量依赖性地引起培养大鼠肝细胞内［Ｃａ２＋ ］ｉ 显著升高；ｒｈＴＮＦ－ＮＣ可使Ｄ－ＧａｌＮ 致敏小鼠出现严重肝损伤，肝组织中Ｃａ２＋ 含量明显增加，钙通道阻滞剂维拉帕米、硝苯地平能显著降低肝组织中Ｃａ２＋ 含量，同时减轻肝损伤程度。结论：ｒｈＴＮＦ－ＮＣ可引起培养大鼠肝细胞及小鼠肝组织中钙稳度失调，钙通道阻滞剂通过减少Ｃａ２＋
Objective: To investigate the effect of rhTNF NC on free cytosolic Ca 2+ concentration ([Ca 2+ ] i) in cultured rat hepatocytes and Ca 2+ contents in mouse livers. Methods: [Ca 2+ ] i was measured by fluorescence spectrophotometer with Ca 2+ detector Fura 2/AM and Ca 2+ contents in mouse livers were examined by atomic absorption spectrophotometer. Results: rhTNF NC dose dependently and significantly increased [Ca 2+ ] i 3 h after it was added into culture medium. rhTNF NC also dramatically increased Ca 2+ contents in mouse livers and induced serious liver injury in mice previously sensitized with D GalN. Calcium antagonists verapamil and nifedipine obviously decreased Ca 2+ contents and attenuated liver injury. Conclusion: Ca 2+ homeostasis in cultured rat hepatocytes and mouse livers is disordered by rhTNF NC. Verapamil and nifedipine can attenuate liver injury induced by rhTNF NC by decreasing Ca 2+ accumulation.
出处
《第二军医大学学报》
CSCD
北大核心
2000年第1期20-23,共4页
Academic Journal of Second Military Medical University
基金
国家新药研究基金!资助项目(96-90154
