摘要
柯楠因是原小檗碱类生物碱类似物.研究发现,在近生理酸度下柯楠因与寡聚腺嘌呤核苷酸(poly A16)形成双链结构,明显增强DNA嵌入染料赛博绿I(SG I)的荧光.在优化条件下,530 nm处荧光增强与60～600 nmol/L的柯楠因呈线性关系,检测限(3σ)为30 nmol/L.方法选择性好,同倍含量的小檗碱类似物不产生干扰.将所建立的方法用于柯楠因合成样的测定,回收率在92.5%～105%之间,相对标准偏差RSD小于3.1%(n=3).
Coralyne is one of the protoberberine alkaloids analogues. It was found that coralyne could induce adenine oligonucleotides （poly A16） to form a double strand DNA under quasi-physiological conditions, greatly enhancing the fluorescence of Sybr Green I （SG I）, a DNA intercalative dye. With the enhanced fluorescence signals of SG I, a new method for the determination of coralyne has been developed in linear range of 60-600 nmol/L, with the detection limit （3σ） of 30 nmol/L. This proposed method is highly selective and other analogues have no interfering effects on the determination, which has been successfully applied for analysis of synthetic samples with the recovery from 92.5% to 105%, and the RSD lower than 3.1%.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2012年第3期352-356,共5页
Acta Chimica Sinica
基金
国家重大新药创制(No.2010ZX09401-306-1-4)
国家自然科学基金(No.21035005)
西南大学基本科研业务费专项基金项目(No.XDJK2009A001)资助项目~~
