摘要
应用斑点杂交和半定量逆转录 多聚酶链反应技术对 3 2例鼻咽癌 (NPC)标本、10例慢性鼻咽炎症粘膜(CINE)标本、NPC细胞株HONE1进行MDM2基因扩增与表达的检测。结果显示 :1例NPC有MDM2基因的扩增 ,NPC细胞株HONE1和 10例NPC有MDM2mRNA高表达 ,CINE无MDM2基因的扩增及mRNA的高表达。MDM2基因的高表达与NPC颈淋巴结转移有关 (P <0 .0 5 ) ,而与NPC的T分期无关。提示MDM2基因在NPC中的主要活化方式是高表达而不是扩增 ,MDM2基因的高表达可能在NPC的发生和转移过程中起着重要作用。
MDM2 gene amplification and overexpression were detected by DNA dot blot analysis and reverse transcription polymerase chain reaction(RT PCR) in 32 cases of nasopharyngeal carcinoma(NPC), 10 cases of chronic inflammation of nasopharyngeal epithelium(CINE) and NPC cell line HONE1. One case of NPC showed MDM2 gene amplification, NPC cell line HONE1 and ten cases of NPC showed MDM2 mRNA overexpression. No MDM2 gene amplification and overexpression were found in CINE. MDM2 mRNA overexpression was significantly related to cervical lymph node metastasis, but not to T stage of NPC. The results indicate that MDM2 gene may play an important role in the pathogenesis and the process of metastasis of NPC.
出处
《湖南医科大学学报》
CSCD
2000年第1期18-20,共3页
Bulletin of Hunan Medical University
关键词
鼻咽肿瘤
MDM2基因
基因扩增
基因表达
nasopharyngeal neoplasm
carcinoma
MDM2 gene
ploymerase chain reaction
