摘要
观察及评价肠内营养（ＥＮ）对治疗短肠大鼠的作用和短肠大鼠结肠形态学代偿性增生的作用。方法：制作切除８０％～８５％的超短肠大鼠模型，用Ｐｅｐｔｉ－２０００作ＥＮ治疗，观察其全身营养状况和结肠形态学的改变。结果：ＥＮ组存活率高达７６．９％，于术后第２１天净氮平衡与对照组无差异，体重仅比术前减轻１０ｇ±１．８ｇ。结肠壁明显增厚，皱襞增大增粗，结肠壁的厚度、粘膜厚度、腺上皮高度和表面积与对照组相比差异非常显著（Ｐ＜０．００１）。ＥＮ组与对照组相比：ＤＮＡ指数１．２１±０．１１比１．０１±０．１５（Ｐ＜０．０５），Ｓ期细胞百分比５２．６％±５．５％比４２．９％±４．１％（Ｐ＜０．０５）。ＥＮ组的ＩＧＦ－１ｍＲＮＡ含量比对照组有极明显增高（１０２６±４２比７３８±４．４，Ｐ＜０．０１）。结论：大鼠结肠在短肠综合征时发生了明显的形态学代偿性增生。早期适当的肠内营养不但可使超短肠大鼠获得足够营养支持，并且能够促进结肠的形态学和功能上的代偿。
To study the adaption of the colon in short bowel rat under enteral nutrition(EN) treatment. Methods: The rats were resected 80% -85% of their small intestine and were treated with Pepti -2000. The nutritional state of the rats and the morphologic changes in the colon were investigated. Results: The survival rate of the EN group was 76.9%. On post-opD21, the net nitrogen gain in the EN group was almost the same as in the CONT group and the body weight was only 10g±1.8g less than the preoperative value. The total thickness of the colonic wall, the thickness of the mucosa, the height and the surface area of the glandular epithelium were greater in the EN group than in the CONT group(p<0.001). The DNA content and the percentage of cells of the S phase were higher in the EN groups than in the CONT group(p<0.05). The levels of colonic mucosal IGF-1 mRNA of the EN group was higher than that of the CONT group(p<0.05). Conclusions: Colonic morphologic and functional adaption occurred in the SBS rats. Early and proper EN not only gives nutritional support to the SBS rats but also enhance colonic adaption to the situation.
出处
《外科理论与实践》
2000年第1期27-30,共4页
Journal of Surgery Concepts & Practice
