摘要
AIM： To investigate the promoter region methylation status of retinoblastoma protein-interacting zinc finger gene 1 （RIZ1） in the human esophageal squamous cell carcinoma （ESCC） cell lines and tissues and verify the relationship between methylation of RIZ1 and oncogen- esis, tumor progression and metastasis etc of ESCC. METHODS： Methylation-specific polymerase chain reac- tion （MSP） was used to investigate the promoter region methylation status of RIZ1 in 6 ESCC cell lines. One cell line where RIZ1 promoter region methylation was de- tected was selected for the next study, where the cell line was treated with 5-aza-CdR. Real-time polymerase chain reaction was used to investigate its influence on the transcription of RIZ1. Experiments using frozenpathological specimens from 47 ESCC patients were performed using the same MSP methodology. RESULTS： Promoter methylation of RIZ1 gene was detected in TEl3, CaEs17 and EC109 cell lines and the cell line TEl3 was chosen for further study. The expression of RIZl mRNA in TE-13 was up-regulated after treatment with 5-aza-CdR. The rate of methyla- tion in carcinomas tissues was significantly higher than those in matched neighboring normal and distal ending normal tissue, and the deviation of data was statisti- cally significant （2,2 = 24.136, P 〈 0.01）. Analysis of the gender, age familial history, tumour deviation, tumour saturation, lymph gland displacement and clinical stag- ing of 47 samples from ESCC patients showed that the fluctuation of data was not statistically significant. CONCLUSION： Promoter methylation may play an im- portant role in the epigenetic silencing of RIZ1 gene expression in human ESCC. RIZ1 is considered to be a potential tumor suppressor gene and may be a biologi- cal parameter for testing early stage human ESCC.
AIM:To investigate the promoter region methylation status of retinoblastoma protein-interacting zinc finger gene 1(RIZ1) in the human esophageal squamous cell carcinoma(ESCC) cell lines and tissues and verify the relationship between methylation of RIZ1 and oncogenesis,tumor progression and metastasis etc of ESCC.METHODS:Methylation-specific polymerase chain reaction(MSP) was used to investigate the promoter region methylation status of RIZ1 in 6 ESCC cell lines.One cell line where RIZ1 promoter region methylation was detected was selected for the next study,where the cell line was treated with 5-aza-CdR.Real-time polymerase chain reaction was used to investigate its influence on the transcription of RIZ1.Experiments using frozenpathological specimens from 47 ESCC patients were performed using the same MSP methodology.RESULTS:Promoter methylation of RIZ1 gene was detected in TE13,CaEs17 and EC109 cell lines and the cell line TE13 was chosen for further study.The expression of RIZ1 mRNA in TE-13 was up-regulated after treatment with 5-aza-CdR.The rate of methylation in carcinomas tissues was significantly higher than those in matched neighboring normal and distal ending normal tissue,and the deviation of data was statistically significant(χ 2 = 24.136,P < 0.01).Analysis of the gender,age familial history,tumour deviation,tumour saturation,lymph gland displacement and clinical staging of 47 samples from ESCC patients showed that the fluctuation of data was not statistically significant.CONCLUSION:Promoter methylation may play an important role in the epigenetic silencing of RIZ1 gene expression in human ESCC.RIZ1 is considered to be a potential tumor suppressor gene and may be a biological parameter for testing early stage human ESCC.
基金
Supported by Grant-in-aid from Specialized Research Fund for the Doctoral Program of Higher Education,No. 20091202110009
Grant-in-aid from Natural Science Foundation of Tianjin,No. 10JCYBJC11300
