摘要
目的探讨卵巢子宫内膜异位症（OEms）异位和在位内膜组织中Survivin的表达及意义。方法应用Elivision^Tm plus免疫组化方法检测30例OEms异位、在位内膜和非子宫内膜异位症子宫内膜组织（对照组）中的Survivin，并应用计算机图像分析系统对结果进行体视学指标积分光密度（IOD）的检测。计算各组内膜组织中的细胞凋亡指数（AI）。、结果OEms异位内膜及在位内膜和对照组中Survivin的IOD分别为（9．87±5．56）、（7．11±4．45）及（3．51±2．54），A1分别为（1．44±O．53）％、（3．10±0．75）％及（4．79±0．69）％，差异均有统计学意义；OEms组和对照组在位内膜分泌期Survivin的IOD高于增生期；Survivin和AI在OEms各组内膜中表达均呈负相关，差异均有统计学意义，在对照组中则无明显相关性。结论Survivin可能影响Ems的细胞凋亡，可能与Eros的发生、发展相关。
Objective To investigate the expression and the significance of Survivin in eutopic and ectopic endometrium of ovarian endometriosis （OEms）. Methods Elivision^Tm plus in imunohisty chemistry method was used to detect Survivin in 30 cases of ectopic endometrium, 30 cases of eutopic endometrium in OEms and 30 cases of endometrium in the normal control. Quantitative images analytical system was performed to detect the integral optical density （IOD） of Suevivin. AI was evaluated in each group by counting ap0ptotic cells on hematoxylin and eosin-stained sections under light mi- croscope. Results IOD of Survivin in ectopic endometrium of OEms, eutopic endometrium of OEms and the normal control were （9.87 ±5.56）, （7.11 ±4.45）, （3.51 ±2.54）, respectively and AI were （1.44 ±0.53） %, （3.10 ±0.75） %, （4.79 ± 0.69） %, respectively. IOD of Survivin in secretory phase of eutopic endometrium of OEms and the normal control was higher than that in the proliferative phase. Survivin and AI had negative correlation, but not in the normal controls. Conclusion Survivin may affect the apoptosis in Ems, and it may be related to the occurrence of Ems.
出处
《中国生育健康杂志》
2012年第1期24-27,共4页
Chinese Journal of Reproductive Health
