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检测肠杆菌科超广谱β-内酰胺酶方法的性能评价

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摘要 目的对临床上如何有效的开展对肠杆菌科超广谱β-内酰胺酶的检测方法进行研究,以利于临床上合理的使用抗生素类药物。方法对送检的58株大肠埃希菌和42株肺炎克雷伯菌分别采用双纸片协同实验、三维实验、纸片表型确证实验和E-test法分别对这100株细菌进行超光谱β-内酰胺酶的检测,记录每种检测方法的灵敏度和特异性。并将结果使用SPSS统计学软件进行χ2检验,α=0.05。结果双纸片协同实验法:敏感性86.21%,特异性88.10%;纸片表型确证方法:敏感性87.93%,特异性85.71%;三维检测法:敏感性93.10%,特异性88.10%;E-test法:敏感性98.28%,特异性95.24%。χ2检验的结果为P<0.05,具有统计学意义。结论四种方法中,E-test法的检测灵敏度和检测特异性最优,对肠杆菌科超广谱β-内酰胺酶的检测效果最佳。
出处 《中外医学研究》 2012年第5期61-62,共2页 CHINESE AND FOREIGN MEDICAL RESEARCH
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