摘要
研究紫山药色素的最佳提取工艺及其抗氧化性能。在单因素试验的基础上,采用L9(34)正交试验法,以pH示差法测定花色苷得率为考察指标,优化了溶剂提取法提取紫山药色素的工艺参数。通过DPPH体系测定该色素清除自由基能力。试验结果表明:紫山药色素属于花色苷类物质,优化的紫山药色素提取条件为:提取温度60℃,提取时间80 min,料液比1∶30,提取溶剂为0.5%盐酸乙醇溶液,提取液花色苷含量可达2.075mg/鲜紫山药g。紫山药色素提取液清除DPPH自由基的IC50为98.14μg/mL。紫山药具有开发功能性色素的潜力。
The extraction technology of pigment from purple yam（Dioscorea alata Linn.sp.） was studied using orthogonal test design.Yield of total anthocyanins through a orthogonal test with pH differential method as an index were investigated in order to get the optimum extraction technology.Furthermore,the antioxidant activity of anthocyanins was analyzed by DPPH assay.The results showed that the optimal extraction conditions were as follows：temperature 60 ℃,extraction duration 80 min,the ratio of material to liquid 1∶30,and the extraction solvent 1.1% HCl ethanol solution and the anthocyanins content arrived 2.075 mg/g in this condition.In addition,the strong DPPH radical scavenging ability（IC50 = 98.14 μg/mL） indicates that purple yam has the potential to be a good functional pigment resource.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2012年第2期229-233,共5页
Natural Product Research and Development
基金
浙江省重大科技项目"薯类加工新产品技术装备研究及产业化开发"(2007C12079)
关键词
紫山药
色素
提取工艺
PH示差法
抗氧化
purple yam
pigment
extraction technology
pH differential method
antioxidant
