摘要
γ-聚谷氨酸(γ-PGA)是一种由枯草芽孢杆菌发酵生产的阴离子聚合物,具有良好的吸水性、生物相容性和生物降解性,无毒性且可由人体降解代谢,是一种很理想的药物载体。发酵得到的γ-PGA一般都具有很高的分子量,本文通过酸水解的方法,得到分子量符合药物载体要求的γ-PGA,并采用离子凝胶法,在水相反应体系中由低分子量γ-PGA与聚赖氨酸(PL)制备得到形态及表面特性良好的PL-γ-PGA纳米颗粒,粒径200～500 nm。在此基础上,制备了搭载葡萄糖脱氢酶(PQQ-GDHs)的PL-γ-PGA纳米缓释系统,载药效率达到61.2%,并且具有很好的稳定性,在体外模拟胃肠道和血液系统的缓释结果表明,分别有31.7%和40.2%的PQQ-GDHs在前48 h被释放。
γ-PGA is a kind of anionic polymer which is produced by microbial fermentation.It has a good capability of water absorption,biodegradation and biocompatibility,also it is non-toxic and can be metabolized in human body.These properties make it an ideal drug delivery.γ-PGA produced by fermentation has high molecular weight.Acid hydrolysis is used to reduce the molecular weight so that γ-PGA could fulfill the requirement of drug delivery.And also,ionic gelation method was applied to make low molecular weight γ-PGA and PL react in aqueous phase to produce novel nanoparticles with good shape and surface characteristic.The diameter of the prepared nanoparticles is about 200~500 nm.These novel PL-γ-PGA nanoparticles were then used to produce the release system of PL-γ-PGA nanoparticles carried PQQ-GDHs.Drug delivered efficiency was stable and about 61.2%.Under the condition of simulated outside gastrointestinal tract and blood system,31.7% and 40.2% PQQ-GDHs were released in first 48 h from the modeling released test,respectively.
出处
《药物生物技术》
CAS
CSCD
2012年第1期35-39,共5页
Pharmaceutical Biotechnology
基金
国家863目标导向项目(No.2006AA02Z239)
