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大鼠脉络膜新生血管色素上皮衍生因子、血管内皮生长因子、血管生成素-2的表达 被引量：3

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摘要 目的探讨大鼠脉络膜新生血管(DNV)组织色素上皮衍生因子(PEDF)和血管内皮生长因子(VEGF)、血管生成素-2(Ang-2)表达,探讨它们在CNV中的作用机制。方法应用氪激光光凝视网膜制备BN大鼠脉络膜新生血管(CNV)模型,随机分为6组,分别在光凝后1,3,7,14,21,28 d行荧光素眼底血管造影(FFA)后摘除眼球。应用实时荧光定量反转录PCR及免疫组化(SP)法检测各组大鼠光凝区和正常视网膜中PEDF、VEGF和Ang-2 mRNA和蛋白的表达,并对结果进行分析。结果光凝后3 d激光损伤区PEDF mRNA和蛋白表达程度最高,与其他各组比较差异显著(均P<0.05),其后表达逐渐下降;光凝后14 d CNV增殖区VEGF表达程度最高,与其他各组比较差异显著(均P<0.05);7 d时Ang-2表达程度最高,与其他各组比较差异显著(均P<0.05)。结论 PEDF表达不足可能是CNV形成和增殖一个主要原因,VEGF与PEDF表达失衡可能在CNV形成过程中起重要调控作用。
作者 崔平 叶存喜 李军会 申景然
机构地区 河北省人民医院医务处 河北医科大学第二医院 石家庄麦格明目眼科门诊部
出处 《中国老年学杂志》 CAS CSCD 北大核心 2012年第4期775-777,共3页 Chinese Journal of Gerontology
关键词 脉络膜新生血管 色素上皮源因子 血管内皮生长因子 血管生成素-2
分类号 R773.4 [医药卫生—眼科]
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参考文献1

二级参考文献10

  • 1Davis S,Aldrich TH,Jones PF,et al.Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning[].Cell.1996
  • 2Suri C,Jones PF,Patan S,et al.Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis[].Cell.1996
  • 3Suri C,McClain J,Thurston G,et al.Increased vascularization in mice overexpressing angiopoietin-1[].Science.1998
  • 4Thurston G,Suri C,Smith K,et al.Leakage-resistant blood vessels in mice transgenically overexpressing angiopoietin-1[].Science.1999
  • 5Maisonpierre PC,Suri C,Jones PF,et al.Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis[].Science.1997
  • 6Holash J,Maisonpierre PC,Compton D,et al.Vessel cooption regression and growth in tumors mediated by angiopoietin and VEGF[].Science.1999
  • 7Lobov IB,Brooks PC,Lang RA.Angiopoietin-2 displays VEGF-dependent modulation of capillary structure and endothelial cell survival in vivo[].Proceedings of the National Academy of Sciences of the United States of America.2002
  • 8Gale N W,Thurston G,Hackett S F,et al.Angiopoietin-2 is required for postnatal angiogenesis and lymphatic patterning, and only the latter role is rescued by Angiopoietin-1[].Developmental Cell.2002
  • 9Jones N,Iljin K,Dumont D J,et al.Tie receptors: new modulators of angiogenic and lymphangiogenic responses[].Nature Reviews Molecular Cell Biology.2001
  • 10Hu B,Guo P,Fang Q,et al.Angiopoietin-2 induces human glioma invasion through the activation of matrix metalloprotease-2[].Proceedings of the National Academy of Sciences of the United States of America.2003

共引文献20

同被引文献82

  • 1Leyla Atmaca,Aysun Idil,Pelin Atmaca-Snmez.Laser treatment in 341 patients with exudative agerelated macular degeneration[J].International Journal of Ophthalmology(English edition),2011,4(1):73-77. 被引量：5
  • 2韩光,韩文东.视网膜色素变性的中医治疗[J].吉林中医药,2006,26(10):41-42. 被引量：18
  • 3吴德银.中医药治疗老年性黄斑变性的临床观察[J].浙江临床医学,2007,9(9):1252-1252. 被引量：5
  • 4LaudeA, CackettPD, VithanaEN, et al.Polypoidal choroidal vasculopathy and neovascular age-related macular degeneration:same or different disease?[J]. Prog Retin Eye Res, 2010, 29(1):19-29.DOI:10.1016/j.preteyeres.2009.10.001.
  • 5HondaS, MatsumiyaW, NegiA. Polypoidal choroidal vasculopathy:clinical features and genetic predisposition[J]. Ophthalmologica, 2013, 35(6):259-264.DOI:10.1159/000355488.
  • 6SuY, ZhangX, ZuoC, et al.Three variants of or near VEGF-A gene are not associated with neovascular age-related macular degeneration and polypoidal choroidal vasculopathy in a Han Chinese population[J]. Ophthalmic Genet, 2015, 36(3):218-223.DOI:10.3109/13816810.2013.858753.
  • 7NakashizukaH, MitsumataM, OkisakaS, et al.Clinicopathologic findings in polypoidal choroidal vasculopathy[J]. Invest Ophthalmol Vis Sci, 2008, 49(11):4729-4737.DOI:10.1167/iovs.08-2134.
  • 8KawamuraA, YuzawaM, MoriR, et al.Indocyanine green angiographic and optical coherence tomographic findings support classification of polypoidal choroidal vasculopathy into two types[J/OL]. Acta Ophthalmol, 2013, 91(6):e474-e481[2015-10-10]. http://onlinelibrary.wiley.com/doi/10.1111/aos.12110.DOI:10.1111/aos.12110.
  • 9RishiP, RishiE, MathurG, et al.Ocular perfusion pressure and choroidal thickness in eyes with polypoidal choroidal vasculopathy, wet-age-related macular degeneration, and normals[J]. Eye (Lond), 2013, 27 (9):1038-1043.DOI:10.1038/eye.2013.106.
  • 10LeeMY, LeeWK, BaekJ, et al.Photodynamic therapy versus combination therapy in polypoidal choroidal vasculopathy:changes of aqueous vascular endothelial growth factor[J]. Am J Ophthalmol, 2013, 156(2):343-348.DOI:10.1016/j.ajo.2013.04.001.

引证文献3

二级引证文献8

中国老年学杂志
2012年 第4期

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