摘要
以普通小麦品种川农16与人工合成小麦衍生品种川麦42为亲本构建了含有127个株系的重组自交系(RIL)群体,将2008和2009年收获的种子用玉米象(Sitophilus zeamais)虫卵进行接种,并于2009年11月开始储藏,次年8月和10月进行虫害调查。利用复合区间作图法(CIM)对RIL群体的抗虫性进行QTL定位分析。在2008年收获群体中检测到位于3B、2D和3D染色体的3个QTL,贡献率依次为10.2%、8.5%和8.3%;在2009年收获群体中检测到位于3D和4B的2个QTL,贡献率分别为8.5%和11.3%。位于3D染色体Xbarc6-Xgwm112标记区间内的QTL在年度间重复检测到,贡献率为8.3%～8.5%,抗性位点来自川农16。
Quantitative trait loci（QTLs） associated with Sitophilus zeamais resistance were identified using a population（127 re-combined inbred lines） from wheat cross Chuannong 16×Chuanmai 42.In a stimulated storage environment,adult,larva,and egg of S.zeamais were artificially fed with wheat seeds harvested in 2008 and 2009,respectively.Five QTLs located on chromosomes 3B,2D,3D,and 4B were detected with phenotypic contributions of 10.2%,8.5%,8.3%,8.5%,and 11.3%,respectively.The QTLs lo-cated between Xbarc6 and Xgwm112 on chromosome 3D were identified in seeds from both growing years,which explained 8.3-8.5% of the phenotypic variance.The positive allele on this locus was from parent Chuannong 16.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第2期369-373,共5页
Acta Agronomica Sinica
基金
国家重点基础研究发展计划(973计划)项目(2011CB100100)资助
关键词
普通小麦
QTL定位
玉米象抗性
Common wheat
QTL mapping
Resistance to Sitophilus zeamais
