摘要
以苯并[a]芘(B[a]P)、甲基亚硝胺吡啶基丁酮(NNK)为目标化合物,设置卷烟烟气冷凝物(CSC)及NNK的质量浓度为0(CK)、0.02、0.04、0.06、0.08、0.10、0.12、0.14、0.16 mg/mL,B[a]P的质量浓度为0(CK)、0.000 8、0.001 6、0.002 4、0.003 2、0.004、0.004 8、0.005 6、0.006 4 mg/mL,研究NNK,B[a]P和CSC对人体支气管上皮细胞(BEAS-2B)的毒性作用。结果表明:CSC的IC50为0.133 mg/mL,NNK为0.110 mg/mL,B[a]P为0.004 6 mg/mL;低剂量B[a]P、NNK分别及联合与CSC作用细胞毒性均大于CSC单独作用,二者分别与CSC作用均表现为协同作用;低剂量CSC、NNK分别及联合与B[a]P作用细胞毒性均大于B[a]P单独作用,二者分别与B[a]P联合作用均表现为协同作用;低剂量CSC、B[a]P分别及联合与NNK作用的细胞毒性与NNK单独作用大小不一,二者分别与NNK作用均表现为拮抗作用;3种毒性物质联合作用均表现为拮抗作用。
B[a]P and NNK were used as the target compounds.CSC and NNK in the dose of 0（CK）,0.02,0.04,0.06,0.08,0.10,0.12,0.14,0.16 mg/mL respectively and B[a]P in the dose of 0（CK）,0.000 8,0.001 6,0.002 4,0.003 2,0.004,0.004 8,0.005 6,0.006 4 mg/mL were tested by MTT to investigate their cytotoxicity on BEAS 2B cells.The result showed the IC50 of cigarette smoke condensates（CSC）,NNK and B[a]P were 0.133,0.110,0.004 6 mg/mL respectively.CSC combined with low dose of B[a]P and NNK respectively or together showed larger cytotoxicity than CSC alone,and CSC with B[a]P and NNK respectively displayed synergism.Low dose of CSC and NNK respectively or together combined with B[a]P showed cytotoxicity greater than B[a]P alone,and CSC and NNK with B[a]P respectively displayed synergism.Low dose of CSC and B[a]P respectively or together combined with NNK showed antagonism.The unification of B[a]P,CSC and NNK showed antagonism.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第1期49-52,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
中国烟草总公司科技项目(110200801022)
关键词
卷烟
烟气
甲基亚硝胺吡啶基丁酮
苯并[A]芘
联合作用
细胞毒性
cigarette
smoke
4（methylnitrosamino）-1（3-pyridyl）-1-butanone（NNK）
benzo[a]pyrene（B[a]P）
synergic
cytotoxicity
