摘要
目的建立高效液相色谱(HPLC)法测定桑姜感冒片中绿原酸的方法。方法采用HPLC法,以EclispseXDBC18(4.6mm×250mm,5μm)为色谱分析柱,乙腈-0.4%磷酸溶液(15∶85)为流动相,流速为0.8mL/min,柱温为30℃,检测波长为326nm。结果绿原酸在0.099～4.990μg范围内呈良好的线性关系(r=0.9998),平均加样回收率为99.63%,RSD=0.59%(n=6)。结论该方法操作简单,结果可靠,重现性及加样回收率等试验项目结果均较为理想,可用于桑姜感冒片中绿原酸的质量控制。
Objective To establish the detecting methods of caffeotannic acid in Ginger cold tablets with the method of high performance liquid chromatography （HPLC）. Methods To detect assaying of caffeotannic acid in Ginger cold tablets with HPLC method, and chromatographic column was Eclispse XDB C,s （4.6 mm×250 mm, 5 μm）, the moving phase was methyl cyanide and phosphoric acid in 0.4% concentration, the flow rate was 0.8 mL/min, the temperature of column was 30℃, and the wave length was 326 nm. Results Linear correlation of caffeotannic acid was stabile from 0.099 to 4.990μg （r = 0.999 8）, recovery rate of sample was 99.63%, RSD = 0.59% （n = 6）. Conclusion HPLC is a simple detecting way, de- tecting consequence is reliably, repeatability is favourable, and the recovery for the sample is perfect, it could be taken in quality control of eaffeotannic acid in Ginger cold tablets.
出处
《中国当代医药》
2012年第6期47-48,共2页
China Modern Medicine
关键词
桑姜感冒片
高效液相色谱法
绿原酸
含量测定
Ginger cold tablets
High performance liquid chromatography （HPLC）
Caffeotannic acid
Determination content
