摘要
目的:研究桂苓神术汤的利尿作用,筛选利尿作用的有效部位并探讨其作用机制。方法:SD大鼠,随机分为18组:正常组、模型组,呋噻米组,桂苓神术汤及石油醚部位、乙酸乙酯部位、正丁醇部位、剩余部位分别设10.0,5.0,2.5 g.kg-1 3个剂量组。每组大鼠均按40 mL.kg-1生理盐水ig,形成盐水负荷模型。45 min后,ig给予不同剂量的桂苓神术汤及不同极性部位,用大鼠代谢笼法观察大鼠6 h的尿量,并测定尿液pH及Na+,K+,Cl-的浓度。结果:桂苓神术汤组(5.0,2.5 g.kg-1),乙酸乙酯部位组(5.0 g.kg-1),剩余部位组(5.0 g.kg-1)均能显著增加盐水负荷大鼠6 h的尿量;尿中K+排出量较模型组均显著升高,分别为(850.3±78.8),(816.6±91.1),(826.3±84.9),(776.7±69.1)mmol.L-1,Na+,Cl-排出量较模型组无显著差异。结论:桂苓神术汤及有效部位具有缓和持久的利尿作用,其利尿作用机制可能为增加大鼠尿液中K+的排泄。
Objective：To study the diuetic effects and its mechanism of Guiling Shenzhu decoction and its different effective parts.Method：The effect on urinre volum and urinary electrolyte（Na＋,K＋,Cl-） were assessed by using rat metabolic cage and electrolyte analyzer following oral administration of Guiling Shenzhu decoction（10.0,5.0,2.5 g·kg-1） and its different polar fractions（10,5.0,2.5 g·kg-1）.Result：Guiling Shenzhu decoction（5.0,2.5 g·kg-1） and extraction of ethyl acetate（5.0 g·kg-1） and residual water layer（5.0 g·kg-1） had good diuretic effect.The urine K＋ excretion increased significantly,but Na＋ and Cl-excretion did not increase.Conclusion：Guiling Shenzhu decoction and extraction of ethyl acetate and residual water layer have significant and persistent diuretic effect.They cause marked increase in urinary K＋level and it may be the diuetic mechanism.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期169-172,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省科技基础设施建设计划项目(BM2008152
BM2008152-KF01)
江苏大学学生科研立项项目(10A236)
关键词
桂苓神术汤
有效部位
利尿
Guiling Shenzhu decoction
effective parts
diuretic activity
