摘要
针对斑马鱼视网膜细胞图像分割时细胞粘连比较严重的情况,采用了边界距离变换和模拟浸水原理的分水岭变换相结合的方法。首先采用距离变换的方法提取细胞区域的局部极小值作为种子点,合并距离过小的错误种子点,然后使用分水岭算法进行分割。实验结果表明,该方法能够有效地分割斑马鱼视网膜细胞图像,成功地解决了分水岭变换中粘连细胞的过分割问题,且计算速度快。
For image segmentation cases of zebra fish retinal cells in serious overlapped, the paper uses the boundary distance transform and the principle of immersion simulation method of combining watershed transform. The basic principle is extracting the local minimum values of ceils as a regional seeds point by using distance transformation. It deletes the error seed points which are too close, and then divided by the watershed algorithm. Experimental results show that the method can effectively split the image of zebra fish retinal cells. The method has successfully solved the over-segmentation problem of watershed transformation in cells adhe- sion, and the calculation is fast.
出处
《微型机与应用》
2012年第4期34-36,共3页
Microcomputer & Its Applications
关键词
粘连细胞
距离变换
分水岭变换
overlapping cells
distance transform
watershed transform