摘要
目的:探讨shRNA介导的神经生长因子β(NGF-β)下调对人胆管癌细胞系QBC939体外增殖与凋亡的影响。方法:构建稳定转染NGF-βshRNA的QBC939细胞系,将细胞分为干扰组(转染NGF-βshRNA组)和对照组(转染空质粒组)。以Western blot方法检测细胞转染效果;细胞增殖实验(CCK-8实验)检测细胞增殖能力,单克隆形成实验检测细胞克隆形成能力;流式细胞术检测细胞周期及细胞凋亡。结果:干扰组细胞较之对照组的细胞增殖、克隆形成能力明显降低,S期细胞明显减少(P<0.05),凋亡率明显增加(P<0.05)。结论:抑制NGF-β能降低QBC939细胞系的增殖和克隆形成能力,该作用与其阻滞细胞进入S期,促进细胞凋亡有关。
Objective： To investigate the effect of shRNA-mediated nerve growth factor β （NGF-β） down-regulation on proliferation and apoptosis of the human cholangiocarcinoma cell line QBC939 in vitro.
Methods： q-he stable QBC939 cells transfected with the NGF-β shRNA were constructed, qhen, the QBC939 cells were divided into interference group （transfected with NGF-β shRNA） and control group （transfected with empty plasmid）. The effect of transfection was determined by Western blot method. The proliferativeand colony formation ability of the Q.BC939 cells was assessed by CCK-8 assay and sphere formation assay, respectively. The cell cycle and apoptosis of the Q.BC939 cells were detected by flow cytometry.
Results： Compared with the control group, the QBC939 cells of interference group presented markedly deceased ability of cell proliferation and colony formation, and significantly reduced ratio of S-phase cells （P〈0.05） and increased apoptotic rate （P〈0.05）.
Conclusion： NGF-β inhibition can decrease the proliferative and colony formation ability of QBC939 cells, which is related to the action of S-phase block and apoptosis enhancement.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2012年第2期158-163,共6页
China Journal of General Surgery
基金
广东省自然科学基金资助项目(9151040701000026)
