摘要
目的探讨黄芪多糖微生态调节作用的活性成分。方法采用水提醇沉法提取黄芪总多糖,三氯乙酸除蛋白,纯化后的多糖分别过中空纤维膜,分子量截留值为150、100、50、20、10和6 kDa,得到不同分子量级别的多糖。应用盐酸林可霉素灌胃建立肠道微生态失调小鼠模型,用不同分子量的7组黄芪多糖进行治疗,同时设正常对照组、阳性对照组和阴性对照组,于给药7 d后处死小鼠,进行各种药效学指标的测定。结果分子量由大到小的7组黄芪多糖占总糖比例依次为59.1%、0.9%、3.4%、9.4%、2.4%、5.3%和19.5%;7组多糖均有不同程度的扶植有益菌、抑制有害菌的作用,其中10～6 kDa多糖调节小鼠肠道微生态菌群平衡效果最好。结论经过药效学实验筛选出10～6kDa黄芪多糖对调节小鼠肠道菌群平衡具有重要作用,初步阐明了黄芪多糖微生态调节作用的物质基础。
Objective To explore the active ingredients of Astragalus polysaccharides（APS） with microecological regulation effects.Method APS were extracted through the methods of water extraction and ethanol extraction.Proteins were removed from APS by trichloroacetic acid（TDA）.Different molecular weight levels of APS were separated by passing through hollow fiber membrane of 150 kDa,100 kDa,50 kDa,20 kDa,10 kDa and 6 kDa.The intestinal microbe dysbiosis mice were established by intragastric administeration of lincomycin,and then treated with 7 different molecular weight levels of APS respectively.And the standard control group,negative control group and positive control group were established.The mice were sacrificed after being treated 7 days.Then various pharmacodynamic indexes were determinated.Result APS in the 7 groups accounted for 59.1%,0.9%,3.4%,9.4%,2.4%,5.3% and 19.5% of the total polysaccharides separately.All the 7 groups showed different levels of supporting the beneficial bacteria and inhibitting the harmful ones,among which the group of 10-6 kDa APS showed the highest effect of regulating the intestinal microbial flora balance of mice.Conclusion 10-6 kDa APS plays an important role in the intestinal microbial flora balance of mice.
出处
《中国微生态学杂志》
CAS
CSCD
2012年第2期113-116,119,共5页
Chinese Journal of Microecology
基金
"973"项目:微生态调节剂防止感染的基础研究(2007CB513008)
关键词
黄芪多糖
微生态调节
物质基础
Astragalus polysaccharides
Microbial ecosystem adjustment
Substance basis
