摘要
用植物胚性细胞团分选仪进行了从胡萝卜非均匀性的悬浮培养物中分级分选体细胞胚。对该仪器分选体细胞胚的操作程序及参数进行了确定,对分离的三个等级的体细胞胚的数量、大小和形态进行了测定。该仪器分选胡萝卜成熟的体细胞胚的速度为每小时从100ml 悬浮培养物中分选出872个胚。按大小次序分级分离的三个等级的体细胞胚所制作的人工种子,在无菌培养基上的萌发率分别为87.3%、75.3%和55.5%。本研究的结果表明植物胚性细胞团分选仪用来分级分选胡萝卜体细胞胚的效果是良好的,成熟胚(第一、二级)制作的人工种子比未成熟胚(第三级)制作的人工种子萌发率有显著的提高。
Carrot somatic embryos were selected fractionally from the cell suspension with an equipm- ent of embryonic cell clumps selector.The operation and optimal conditions of this equipment used for selection of somatic embryos(SEs)have been tested and determined repeatedly.Three classes of SEs in different sizes,numbers and morphology were obtained.The rate of fractional selection with this equipment was 872 mature embryos/100 ml cell suspension/h. These 3 classes of selected SEs were encapsulated in alginate beads to produce artificial seeds. In vitro germination frequencies of these seeds with production of seedling-quality plantlets were tested on MS medium in sterilized condition.In addition seedlings from artificial seeds grew normally to adults in soil pots.These results indicated that the use of selected SEs to produ- ce artificial seeds increases the seed quality obviously.
基金
863高技术发展计划资助课题
关键词
胡萝卜
体细胞胚
人工种子
Artificial seed
Somatic embryo
Carrot
