摘要
风信子(Hyacinthus orientalis)雄蕊再生的最适温度是25℃。小孢子发生和花粉发育是在温度不断下降的过程中完成的。母细胞分化的最适温度是20—25℃;减数分裂的最适温度是20℃;花粉第一次有丝分裂的最适温度是10℃。按这样的温度条件能够将再生雄蕊培养成熟,并且成熟雄蕊的花粉具有较高的萌发率。不合适的温度将导致小孢子发生和花粉发育停止在某个阶段,最终花粉败育。
In order to culture the regenerated stamens of hyacinth in vitro to maturity,the effects of temperature on regeneration of stamens,microsporogenesis and pollen development were studi- ed.Results showed:the proper temperature for stamen regeneration was 25℃.The tempe- rature going down gradually was advantageous to the microsporogenesis and pollen develop- ment.The most suitable temperatures for differentiation of microsporocyte,meiotic division and pollen first mitosis were 20—25℃,20℃ and 10℃ respectively.Under such temperature condition,it was possible to culture the regenerated stamens to maturity,and pollen grains in the stamens had the higher germination frequency.On the contrary,unsuitable temperature condition will make microsporogenesis and pollen development stop at certain development stage, it will finally result in pollen abortion.
基金
国家自然科学基金资助课题
关键词
风信子
温度
雄蕊再生
小孢子
花粉
Hyacinthus orientalis L
Temperature effect
Stamen regeneration
Microsporogenesis
Pollen development
