摘要
以菲牛蛭为原料,采用Alcalase 2.4 L碱性蛋白酶酶解菲牛蛭制备抗凝血肽,通过筛选显著单因素和设计正交试验,并以抗凝血活性为指标,确定酶解的最佳反应条件为:酶解温度55℃、酶解时间2 h、底物浓度12%、加酶量3%、pH 8.5,在该条件下所制备的抗凝血肽的抗凝血活性可达702AT-U/g。
In order to obtain high activity ancoagulant peptide from Poecilobdella manillensis Lesson, an enzymolysis method was established through sequential steps of screening single-factor and designing orthogonal test. By selecting ancoagulant activity as index, the optimum enzymolysis reaction conditions were determined as enzymolysis temperature 55℃, enzymolysis temperature 2h, substrate concentration 12%, enzyme dosage 3% and pHS.5. The ancoagulant activity could reach 702AT-U/g under the optimized conditions.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第2期164-167,155,共5页
Modern Food Science and Technology
基金
新世纪优秀人才支持计划项目(NCET-060746)
关键词
菲牛蛭
抗凝血活性
ALCALASE
2.4L
抗凝血肽
Poecilobdella manillensis Lesson
ancoagulant activity
Alcalase 2.4L
ancoagulant peptide
