摘要
离体黄瓜(Cucumis sativus L.)子叶在6-BA(10ppm,黑暗条件)诱导下发生明显的膨大。利用α-微管蛋白和β-微管蛋白的 cDNA 为探针,研究了6-BA 诱导下子叶膨大过程中微管蛋白基因的转录活性。结果表明在6-BA 作用下,离体黄瓜子叶中徽管蛋白两个亚单位的基因被迅速地激活。这说明微管蛋白基因表达的增强与子叶膨大这一生理现象密切相关.
The expansion of excised cucumber cotyledons was induced by the treatment of 10 ppm of 6-benzyladenine(6-BA)in dark.The elongation and division of ceils in this cotyledon are related to the activation of microtubules.The cDNAs of α-and β-tubulin from mouse and Phvsarum polycephalum were used as probes to deect the transcriptional regulation of tubulin genes in the expanding cotyledons treated with 6-BA.The expression of tubulin genes has also been analyzed at the translational level by Western blot and ELISA with antibody against tubulin purified from chicken brain.The results showed that genes encoding two subunits of tululin in excised cucumber cotyledons were rapidly activated by cytokinin 6-BA at both transcriptional and translational levels.It implied that tubulin increasement was involvedim the cytokinin-induced expansion of excised cucumber cotyledons.
基金
国家自然科学基金
关键词
黄瓜
细胞分裂素
微管蛋白基因
Cytokinin
Tubulin
Cucumber cotyledon
Gene expression
