摘要
本文对乌鞘岭地区天然分布的中国沙棘(Hippophae rhamnoides L.subsp sinensis)和西藏沙棘(H.thibetana schlechted)物候期进行了观察,对它们的果实生长与成熟期间各种植物激素含量水平的变化进行了系统测定。结果表明:种子内的 GA_3出现了两次高峰值(花后105—109天和128—132天);玉米素在果实开始变色时出现(花后92—96天),这时种子内iPA 测出第一高峰值;果实完熟开始落叶时,iPA 出现第二高峰值(花后127—136天),玉米素高峰值也与此接近(花后127天)。种子内的玉米素减少时,果柄与果肉中其含量仍很高。GA_3、异戊烯基腺苷(iPA)的第一高峰值与 ABA 出现时间接近,GA_3、iPA、IAA 第二高峰出现时间与 ABA 及玉米素高峰值重合。表明在果实生长与成熟过程中,植物激素的平衡状态比单个激素的绝对含量更为重要,同时也证明在沙棘果成熟与变色过程中 GA_3与 CTK 具有重要的调节功能。
The phenological stages in seabuckthorn (Hippophae rhamnoides L.Subsp.sinensis Rousi and H.thibetana Schlechted) distributed naturally over an area of Wushao ridge were obser- ved.The changes in levels of endogenous phytohormones GA_3,iPA,zeatin,IAA and ABA were investigated systematically during development and ripening periods in the two sorts of fruits. The results showed that two peak values of GA_3 level in seeds were detected (in 105-109 days and 128-132 days after anthesis).The first peak value of isopentenyl adenine (iPA) in seeds was determined in 92-96 days when the fruit was starting to change its colour and just right now the zeatin first appeared.The iPA content reached the second peak value in 127- 136 days after anthesis during fruit full ripening and beginning to deciduous leaf and the ma- ximum of zeatin in seeds appeared near the same time (in 127 days after anthesis).It was remarkable that the zeatin content in fruit stalk and flesh still was kept on high level,in the meantime the zeatin level in seeds dropped down rapidly.Based on the facts that the time of the first peak value in GA_3 and iPA level is close to the date when ABA is first detected,and the date of second peak value in GA_3,iPA and IAA is coincident with the time of maximum in ABA and zeatin levels make it reasonable to consider that the balance of phytohormones may be more important than absolute amounts of any single hormone during the periods of fruit development and ripening,and meanwhile it also proved that GA_3 and CTK play an important regulatory role in controlling fruit ripening and colour changing process of seabuckthorn fruit.
基金
国家自然科学基金资助课题
