摘要
本文报道了松科植物青杆(Picea wilsonii Mast)的合子胚在固体和液体悬浮培养的条件下产生大量体细胞胚,并在固体培养基上进一步发育成苗的研究结果。青杆球果采自中国科学院北京植物园,保存在 4—6℃中两个月。取幼胚于含 3.4％蔗糖、2.2mg/l 2,4-D和 1.1mg/l BA的改良LP固体培养基上,在黑暗中培养两个月。幼胚产生的白色和淡褐色的愈伤组织再转入含 1.0mg/l KT和 1.0mg/l 2,4-D的改良 59固体培养基上。经一个月的培养,原相似的愈伤组织可明显地分为三种类型:1.
Embryogenic callus (translucent callus) was produced from immature zygotic embryos of Picea wilsonii Mast. Subsequently somatic embryogenesis occurred on the brown callus. The somatic embryos could be stimulated to developinto plantlets on the medium without hormone. Young somatic embryos were produced from embryogenic callus in liquid suspension culture, in which suspensor was several or more than ten times the size of the somatic embryo. The somatic embryo showed very similar to zygotic embryos in micro--section and living material.
关键词
青qian
体细胞胚发生
悬浮培养
Gymnosperm
Picea wilsonii Mast
Somatic embryogenesis
Suspension culture
