摘要
莲(Nelumbo nucifera Gaertn.)幼胚子叶细胞造淀粉体和分离的造粉质体上均呈现强烈的 Feulgen 反应物质。经 DNA 酶处理后,在子叶细胞造淀粉体上呈 Feulgen 负反应。将分离的造粉质体用特异性的 DNA 荧光染料 DAPI 染色,造粉质体显示蓝色的荧光。实验证明,莲幼胚子叶细胞随着发育时期的增长,造粉质体 DNA 的含量逐渐增加,显示出有规律的动态变化过程。在电镜下观察,造粉质体 DNA 区域外无膜结构存在,故具有原核生物的特征。这种质体 DNA 的纤维丝直径大约为25。
It was first shown that the amyloplasts in young cotyledon cells of lotus (Nelumbo nuci- fera Gaertn.) and the isolated amyloplasts stained with Feulgen reaction and treated by DNase, had Feulgen-positive reaction and Feulgen-negative reaction respectively.The isolated amylo- plasts stained with DAPI demonstrated blue fluorensence.Some experiments proved that DNA content in the amylopasts increased gradually with the development of cotyledon,showing the dynamic change of DNA in the amyloplasts.Electron microscopic observation revealed that there was no membrane surrounding the DNA region of the amyloplasts illustrating the characte- ristic of its protokaryotic organisms.The DNA fibrils were about 25 in diameter.
关键词
莲
胚
子叶细胞
造淀粉体
DNA
Young cotyledon cells of lotus
Feulgen reaction
Amyloplast DNA
