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诱导物对出芽短梗霉木聚糖酶活力和阿魏酰低聚糖合成的调控影响 被引量:4

Effect of Inducers on Xylanase Activity and Biosynthesis of Ferulic Oligosaccharides in Aureobasidium pullulans
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摘要 以麦麸为原料诱变选育的不产黑色素出芽短梗霉为发酵菌株,利用其发酵过程中合成的木聚糖酶水解麦麸纤维,制备阿魏酰低聚糖(FOs)。研究碳源、氮源、金属离子和表面活性剂等诱导物对出芽短梗霉木聚糖酶活力和FOs合成的影响,以探明各物质对出芽短梗霉木聚糖酶活力和FOs合成的影响;利用正交试验设计方法研究各物质添加量对出芽短梗霉产酶和FOs的影响,确定芽短梗霉发酵制备FOs的最佳培养基。结果表明:50g/L麦麸处理液中添加15g/L葡萄糖、1g/L蛋白胨和1g/L玉米浆,FOs产量和木聚糖酶活力最高分别达到627nmol/L和52.66IU/mL。 Feruloyl oligosaccharides(FOs) could be produced as a result of the hydrolysis of wheat bran fiber by xylanase synthesized during fermentation by a mutant strain of Aureobasidium pullulans without the secretion of melanin bred using wheat bran.The effects of inducers such as carbon source,nitrogen source,metal ion and surfactant on the activity of xylanase and the formation of FOs were investigated.The medium composition for FOs production by the strain was optimized using the orthogonal array design method.The highest Fos production and xylanase activity,627 nmol/L and 52.66 IU/mL,respectively,were achieved by using a medium comprised of 50 g/L wheat bran,15 g/L glucose,1 g/L peptone and 1 g/L corn syrup.
出处 《食品科学》 EI CAS CSCD 北大核心 2012年第1期209-214,共6页 Food Science
基金 江苏省社会发展科技支撑基金资助项目(BE2011728) 江苏省高校自然科学基金资助项目(11KJB550005) 江苏省农业科技支撑基金资助项目(BE2010386) 江苏省新型环保重点实验室开放课题基金资助项目(AE201041) 盐城市产学研联合创新基金项目(YKA201102) 盐城市科技局工业支撑基金资助项目(Yk2009047) 盐城工学院应用化学研究所开放基金资助项目(XKY2009004)
关键词 麦麸 阿魏酰低聚糖 诱导物 合成调控 出芽短梗霉 wheat bran ferulic oligosaccharides inducer biosynthesis and control Aureobasidium pullulans
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参考文献24

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