摘要
采用农杆菌介导的方法,把CaMV35S启动子驱动的来自棉花(Gossypiumspp.)的抗坏血酸过氧化物酶(ascorbate peroxidase,APX)基因导入普那菊苣(Cichorium intybus L.cv.Puna)。结果表明:经过卡那霉素(Km)筛选和对抗性植株的PCR和Southern杂交分析,证明APX基因成功地整合到普那菊苣基因组中。转APX基因普那菊苣植株对NaCl和甘露醇胁迫表现出一定的抗性,在NaCl浓度为500mmol.L-1、甘露醇浓度为30g.L-1的条件下,转APX基因不定芽能够正常生根和生长,转基因植物叶片外植体能够形成愈伤组织和再生植株,而野生型植株不定芽不能正常生根、已生根幼苗不能正常成长,野生型植株叶片不能形成愈伤组织。
The cotton APX gene was transferred into Cichorium intybus L.cv.Puna by Agrobacterium-mediation.The transgenic chicory plants were first screened by Kanamycin then detected by both PCR and Southern blot approaches.Effects of both sodium chloride and mannitol on chicory calli and seedling growth were studied.Results indicated that transgenic Puna chicory explants could formed calli and grew into normal seedlings on medium containing 500 mmol？？L-1 NaCl and 30 g·L-1 mannitol,while non-transgenic explants could not form callus on the same medium.
出处
《草地学报》
CAS
CSCD
北大核心
2012年第1期152-158,共7页
Acta Agrestia Sinica
基金
国家转基因生物新品种培育重大专项(2009ZX08003-017B)
山西省农科院攻关项目(Ygg0912)资助
关键词
普那菊苣
农杆菌介导
遗传转化
抗逆性
Cichorium intybus L. cv. Puna
Agrobacterium-mediated
Genetic transformation
Stress tolerance
