摘要
从花椰菜的无菌苗下胚轴游离原生质体经纯化后的得率为1.5～2×10~6/g FW。通过液体浅层培养、平板固体培养、双层培养和gelrlte包埋培养方法的比较,发现gelrite包埋培养法,最有利于花椰菜下胚轴的原生质体培养。纯化的原生质体用MS-1培养基培养,再生细胞的分裂频率为24％。再生的愈伤组织转到分化培养基MS-5A或MS-5B上迅速分化出苗。共获得再生植株78株,移栽到盆中生长正常,结出正常的菜花。
Hypocotyl protoplasm were isolated from sterile seedlings of cauliflower (Brassica oleracea var. botrytis L.). After the purification of protoplasts, a high protoplast yield (1.5～2×10~6 g^(-1) FW) was obtained. The purified protoplasm were cultured in MS-1 medium. It was observed that the division frequency of the regenerated cells was about 240A. It indicated that gelrite embedding culture floating in liquid medium is the most suitable one for the hypocotyl protoplasts of catuliflower in the culture methods tested, which include thin liquid culture, gelrite solid layer culture and liquid over gelrite double layer culture. Intact plantlets quickly differentiated from the regenerated calli after they were transferied onto a differentiation medium (MS-5A or MS-5B). 78 plantlets were regenerated from3 cultivars, and normal cauliflowers were obtained after the transplantation of the plantlets into pots.
基金
"七五"国家攻关资助项目
关键词
花椰菜
下胚轴
原生质体培养
Brassica oleracea var. botrytis., hypocotyl, protoplast culture, plant regeneration
