摘要
植物细胞包括原生质体的生长,都依赖于细胞壁的伸展。而壁结合伸展素又是影响细胞壁伸展最直接的因素,Lamport(1965,1967)报道羟脯氨酸残基通过O-阿拉伯糖苷键与壁中其他多聚体相连,形成网络,调节壁伸展。Fry(1982,1984)分离到一种异联酪氨酸后。
The relation of hydroxylase and covalently wall-bound peroxidase with the cell expansion of cucumber cotyledons was investigated,
The excised and aerated cotyledons of cucumber grew in basal medium (1/15 mol/L Na^+-K^+ phosphate buffer conraining 10 ppm 6-BA, pH 6.0 ) for 36 h in the dark. The covalently well-bound peroxidase activity increased with the increase of cell expansion of cucumber cotyledons (Fig. 1).
α, α'-dipyridyl is an inhibitor of hydroxylase. At the concentrations used, i. e., 0.1, 1 and 10 mmol/L, it inhibited both the activity of covalently wall-bound peroxidase and cell expansion of cucumber cotyledons (Table 1).
It was shown that when ascorbate was used as an inhibitor of peroxidase, the cell expansion of cucumber cotyledons was inhibited along with the inhibition of peroxidase activity (Table 2).
Therefore, the covalenfly wall-bound peroxidase possesses the function of peptidylproline-hydroxylase, and proline hydroxylation is essential to cell expansion.
The results of treatment with exogenous IAA showed an interaction between peroxidase and IAA.
基金
国家自然科学基金
关键词
黄瓜
子叶
过氧化物酶
cucumber cotyledon, covalently wall-bound peroxidase, α, α'-dipyridyl, ascorbate, IAA
