摘要
次第选用固体培养基、有机培养溶液和无机培养溶液,通过改善培养条件,从凤眼莲的腋芽培养出生长正常的无菌苗。用生物检测法测试无菌苗对衣藻的克制效应。试验结果表明,在无根际微生物着生的条件下,凤眼莲种植水仍然显示了克藻作用,说明克藻物质是由凤眼莲本身分泌的,不是由根际微生物所产生的。
After testing organic solid, organic liquid and inoraganic liquid culture media one by one and improving culture conditions, sterilized seedlings of water hyacinth (Eichhornia crassipes Solms.) with normal growth were established from axillary buds. A bioassay test was conducted to examine the allelopathic effect of the sterilized seedlings on Chlamydomonas reinhardtii. The results indicated that the extract from the water in which water hyacinth had been cultured under sterilized conditions still showed allelopathic effect. It means that the allelopathic compounds were exuded by water hyacinth itself, not by microbes living in its root zone.
关键词
凤眼莲
无菌苗培养
克藻效应
water hyacinth, sterilized culture., Chlamydomonas reinhardtii, allelopathic effect
