摘要
目的:考察阿托品输液的稳定性,探讨阿托品输液法抢救有机磷中毒患者的可行性。方法:建立RPHPLC法[色谱柱为NovaPakC184μm,3.9×150mm,流动相为甲醇/水(28∶72)]加入三乙胺0.12%,并用冰醋酸调pH5.0,检测波长210nm,流速0.8ml·min-1,柱温40℃,氢溴酸山莨菪碱做内标,检测临床应用条件下阿托品含量变化。另测定pH、不溶性微粒及外观变化。结果:在临床应用条件下阿托品含量、pH、外观24h稳定。经临床应用,阿托品输液法抢救有机磷中毒29例效果满意。结论:阿托品输液法抢救有机磷农药中毒是切实可行的。这对于中度、重度中毒患者减少被反复穿刺的痛苦。
OBJECTIVE:This paper was to observe the stability of atropine sulfate in normal infusion and to serve basical data for clinical application.METHODS:Atropine sulfate 0.1 or 1.0 mg·ml -1 was combined with infusion.The concentration of atropine was determined by an established RP HPLC.A Nova Pak C 18 column ( 3.9 ×150 mm) and 28% methanol in water and 0.12% triethylamine with acetic acid adjusting pH 5.0 as the mobile phase and the flow rate of 0.8 ml·min -1 and column temp.Of 40℃ were adopted and the detection wavelength of 210nm was specified.Anisodamini hydrobromidium used as an internal standard.The solution were visually assessed for color and clarity,and particulate content was measured with a particle counter,and pH was measured.RESULTS:All solutions containing atropine retained more than 90% of their initial levels.pH and appearance had no significant change.The consequence of clinical application is satisfying in 29 patients of organic phosporous poisoning.CONCLUSIONS:Atropine sulfate was stability in normal infusion for 24h and used by infusion was feasible.It has clinical implication to the medial or severe organic phosporous poisoner.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2000年第2期82-83,共2页
Chinese Journal of Hospital Pharmacy
关键词
RP-HPLC
阿托品
稳定性
输液
有机磷中毒
RP HPLC
atropine sulfate
stability
infusion
organic phosporous poisoning
