摘要
为了解海洛因依赖者ＴＴ 病毒感染状况、研究其致病性及病毒特征，探讨感染传播途径，采用巢式ＰＣＲ 和ＥＬＩＳＡ 法检测２８６ 例海络因依赖者血清ＴＴ病毒ＤＮＡ 和抗体ＩｇＧ。结果显示：２８６ 例血清标本ＴＴ 病毒ＤＮＡ 和抗体ＩｇＧ的阳性率分别为７－０ ％ 和９－４％ ；无一例标本同时检出ＴＴ 病毒ＤＮＡ 和抗体ＩｇＧ；ＡＬＴ 升高和正常组ＴＴ 病毒感染阳性率差异无显著性（ Ｐ＞０－０５） 。结果显示：海洛因依赖者存在ＴＴ 病毒感染和无症状携带状态，感染不依赖其它肝炎病毒而单独存在；ＴＴ病毒可能是一种新的自限性ＤＮＡ病毒，其对肝脏的损害作用不确切；静脉注射不是海洛因依赖者ＴＴ病毒感染的主要途径。
To understand the infective status and study the pathogenic role of TT virus and the viral characteristics,and elucidate the pathway of transmission of TT virus in heroin addicts,nested-polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA) were used to detect TT virus DNA and anti-TT virus IgG in 286 sera samples from patients.The positive rates of TT virus DNA and anti-TT virus IgG were 7 0% and 9 4%;respectively.None sample was positive both TT virus DNA and anti-TT virus IgG.The positive rates of TT virus DNA were no significant difference between in the elevated and in the normal alanine aminotransferase(LAT) levels groups(χ 2=0 054,P>0 05).There are TT virus infection and asymptomatic status in the heroin addicts,the infection of independent of other hepatitis virus could exist alone.TT virus is probably a new self-limited DNA virus and the biochemical or the histological evidence of significant liver damage were not seen.TT virus may not be transmitted mainly by infection of vein in heroin addicts.
出处
《中国医师杂志》
CAS
2000年第1期24-25,28,共3页
Journal of Chinese Physician
关键词
TT病毒
海洛因依赖
PCR
ELISA
TT virus *
Heroin addicts
Polymerase chain reaction
Enzyme-linked immunosorbent assay
