摘要
目的探讨Ｄｕｂｉｎ－Ｊｏｈｎｓｏｎ综合征（Ｄｕｂｉｎ－Ｊｏｈｎｓｏｎ ｓｙｎｄｒｏｍｅ，ＤＪＳ）病人肝细胞内色素颗粒的本质。方法应用光镜、组织化学、免疫组织化学和电镜技术对肝细胞内色素颗粒进行探讨及其对Ｓ—１００蛋白和ＨＭＢ４５的表达。结果组织学检查显示肝小叶结构正常，肝细胞内有大量棕褐色颗粒沉着，尤以小叶中央区明显。组织化学染色和超微结构观察结果证实色素颗粒具有脂褐素和黑色素的特征，免疫组织化学染色证实为黑色素。结论ＤＪＳ病人肝细胞内色素颗粒为脂褐素一黑色素复合物。
Objective To investigate the property of pigment granules in the hepatocytes in patients with DubinJohnson syndrome. Method Light microscopy, histochemical, immnohistochemical and electron microscopy techniques were used to study the pigment glanules and the expression of S-100 protein and HMB45 in hepatocytes. Results Histological examination revealed normal lobular architecture and the abundont brown pigments which were chief'ly seen in the centrilobular zone hepatocytes. The pigment granules were evidenced to have the characterization of both lipofuscin and melanin by histochemical staining and ultrastructural studies and to have the featurts of the melanin by immnohistochemical staining. Conclusions The results suggest that the pigment grannies are lipfuscin-melanin complex in the hepatocytes in patients with Dubin-Johnson syndrome.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第1期45-47,共3页
Chinese Journal of Hepatology
关键词
免疫组织化学
病理学
超微结构
DJS综合征
Jaundice, chronic iniopathic
Histochemistry
Immnohistochendsny
Pathology, clinical
Ultrastructure
